Western Blot (WB)
(Western Blot: Sample: Recombinant TFR, Human)
Mouse Transferrin Receptor (TFR) Monoclonal Antibody | anti-TFR antibody
Monoclonal Antibody to Transferrin Receptor (TFR)
Immunohistochemistry: 5-50ug/mL
Immunocytochemistry: 5-50ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant TFR, Human)
Western Blot (WB)
(Western Blot: Sample: Recombinant TFR, Human)
Western Blot (WB)
(Western Blot;Sample: Lane1: Hela cell lysate;Lane 2: MCF7 cell lysate; Lane 3: K562 cell lysatePrimary Ab: 0.4ug/ml Mouse Anti-Human TFR AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035))
Western Blot (WB)
(Western Blot;Sample: Lane1: Hela cell lysate;Lane 2: MCF7 cell lysate; Lane 3: K562 cell lysatePrimary Ab: 0.4ug/ml Mouse Anti-Human TFR AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Liver Tissue;Primary Ab: 40ug/ml Mouse Anti-Human TFR AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Liver Tissue;Primary Ab: 40ug/ml Mouse Anti-Human TFR AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Small intestine Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Small intestine Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Uniprot Description
TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.
Protein type: Membrane protein, integral; Cell surface; Receptor, misc.
Chromosomal Location of Human Ortholog: 3q29
Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; vesicle; endosome; external side of plasma membrane
Molecular Function: identical protein binding; protein binding; transferrin receptor activity; double-stranded RNA binding
Biological Process: receptor-mediated endocytosis; viral reproduction; cellular iron ion homeostasis; positive regulation of bone resorption; transferrin transport; regulation of cell growth; osteoclast differentiation; transmembrane transport; regulation of cell proliferation
Research Articles on TFR
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Product Notes
The TFR tfrc (Catalog #AAA2001263) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Transferrin Receptor (TFR) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Transferrin Receptor (TFR) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-5ug/mL Immunohistochemistry: 5-50ug/mL Immunocytochemistry: 5-50ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the TFR tfrc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transferrin Receptor (TFR), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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