Human Pleiotrophin (PTN) CLIA Kit | PTN clia kit

Human Pleiotrophin (PTN) CLIA Kit

Cat. Num. AAA2000638

• Gene Names: PTN; HARP; HBNF; HBGF8; NEGF1
• Reactivity: Human
• Synonyms: Pleiotrophin (PTN); Human Pleiotrophin (PTN) CLIA Kit; HARP; HBGF8; HBNF; NEGF1; Heparin Binding Growth Factor 8; Neurite Growth-Promoting Factor 1; Heparin Affin Regulatory Peptide; Heparin Binding Growth Associated Molecule; PTN clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pleiotrophin (PTN).; No significant cross-reactivity or interference between Pleiotrophin (PTN) and analogues was observed.
• Sequence Length: 168

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 2.74-2,000pg/mL
• Sensitivity: < 0.95pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTN clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Pleiotrophin (PTN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pleiotrophin (PTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pleiotrophin (PTN) level in the sample or standard.;

Product Categories/Family for PTN clia kit

Cytokine; Tumor immunity; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 4506281
• NCBI GeneID: 5764
• NCBI Accession #: NP_002816.1
• NCBI GenBank Nucleotide #: NM_002825.5
• UniProt Accession #: P21246; Q5U0B0; Q6ICQ5; Q9UCC6
• Molecular Weight: 18,942 Da
• NCBI Official Full Name: pleiotrophin
• NCBI Official Synonym Full Names: pleiotrophin
• NCBI Official Symbol: PTN
• NCBI Official Synonym Symbols: HARP; HBNF; HBGF8; NEGF1
• NCBI Protein Information: pleiotrophin
• UniProt Protein Name: Pleiotrophin
• Protein Family: Pleiotrophin
• UniProt Gene Name: PTN
• UniProt Synonym Gene Names: HBNF1; NEGF1; PTN; HBBM; HBGF-8; HB-GAM; HBNF-1; OSF-1
• UniProt Entry Name: PTN_HUMAN

Uniprot Description

PTN: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti- apoptotic signaling of PTN and regulation of cell proliferation. Belongs to the pleiotrophin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: extracellular space; endoplasmic reticulum

Molecular Function: heparin binding; growth factor activity; protein phosphatase inhibitor activity

Biological Process: nervous system development; positive regulation of cell division; negative regulation of catalytic activity; positive regulation of cell proliferation; learning; transmembrane receptor protein tyrosine phosphatase signaling pathway; bone mineralization