Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1) CLIA Kit | IGF2BP1 clia kit

CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1)

Cat. Num. AAA2000469

• Gene Names: IGF2BP1; IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
• Synonyms: Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1); CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1); IGF2BP1 clia kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1); No significant cross-reactivity or interference between Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1) and analogues was observed.
• Sequence Length: 577

• Assay Type: Sandwich
• Samples: Tissue homogenates and other biological fluids
• Detection Range: 0.014-10ng/mL The standard curve concentrations used for the ELISA's were 10ng/mL, 3.33ng/mL, 1.11ng/mL, 0.37ng/mL, 0.123ng/mL, 0.041ng/mL, 0.014ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.059ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Organism Species: Homo sapiens (Human)
• Assay Length: 4.5 hours
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF2BP1 clia kit

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1) level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 56237027
• NCBI GeneID: 10642
• NCBI Accession #: NP_006537.3
• NCBI GenBank Nucleotide #: NM_006546.3
• UniProt Accession #: Q9NZI8; C9JT33
• Molecular Weight: 63,481 Da
• NCBI Official Full Name: insulin-like growth factor 2 mRNA-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: insulin-like growth factor 2 mRNA binding protein 1
• NCBI Official Symbol: IGF2BP1
• NCBI Official Synonym Symbols: IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 1; ZBP-1; VICKZ family member 1; zipcode-binding protein 1; IGF2 mRNA-binding protein 1; IGF-II mRNA-binding protein 1; coding region determinant-binding protein
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 1
• Protein Family: Insulin-like growth factor 2 mRNA-binding protein
• UniProt Gene Name: IGF2BP1
• UniProt Synonym Gene Names: CRDBP; VICKZ1; ZBP1; IGF2 mRNA-binding protein 1; IMP-1; IMP1; CRD-BP; ZBP-1
• UniProt Entry Name: IF2B1_HUMAN

NCBI Description

This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

IGF2BP1: a conserved trans-acting factor that can control translation of specific mRNAs in vivo. Contains four K homology (KH) domains and two RNA recognition motifs. It functions by binding to the 5' UTR of the target mRNAs and repressing translation. Target mRNAs include those encoding beta-actin and insulin-like growth factor 2 (IGF2). Tyrosine phosphorylation apparently abolishes translational repression of actin expression by decreasing its binding beta-actin mRNA. May have a role in progression of ovarian cancer. Contains nuclear export signals within the RNA-binding KH2 and KH4 domains. Aberrant expression may interfere with c-myc regulation.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: growth cone; axon; stress granule; perinuclear region of cytoplasm; lamellipodium; cytoplasm; dendritic spine; cytosol; nucleus; ribonucleoprotein complex; filopodium

Molecular Function: mRNA binding; protein binding; mRNA 3'-UTR binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: mRNA transport; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process