Zebrafish Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit | TGFbI elisa kit

Zebrafish Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit

Cat. Num. AAA2000272

• Gene Names: TGFBI; CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
• Reactivity: Zebrafish
• Synonyms: Transforming Growth Factor Beta Induced Protein (TGFbI); Zebrafish Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit; ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI); TGFbI elisa kit

• Reactivity: Zebrafish
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta Induced Protein (TGFbI).; No significant cross-reactivity or interference between Transforming Growth Factor Beta Induced Protein (TGFbI) and analogues was observed.
• Sequence Length: 683

• Samples: Serum, plasma, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 7.813-500 pg/mL The standard curve concentrations used for the ELISA's were 500 pg/mL, 250 pg/mL, 125 pg/mL, 62.5 pg/mL, 31.25 pg/mL, 15.625 pg/mL, 7.813 pg/mL
• Sensitivity: Typically less than 3.8 pg/mL.
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta Induced Protein (TGFbI) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta Induced Protein (TGFbI) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.

Related Product Information for TGFbI elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Induced Protein (TGFbI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta Induced Protein (TGFbI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta Induced Protein (TGFbI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta Induced Protein (TGFbI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 339568
• NCBI GeneID: 7045
• UniProt Accession #: Q15582; O14471; O14472; O14476; O43216; O43217; O43218; O43219; Q53XM1; D3DQB1
• Molecular Weight: 74,681 Da
• NCBI Official Full Name: transforming growth factor induced protein
• NCBI Official Synonym Full Names: transforming growth factor, beta-induced, 68kDa
• NCBI Official Symbol: TGFBI
• NCBI Official Synonym Symbols: CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
• NCBI Protein Information: transforming growth factor-beta-induced protein ig-h3; RGD-CAP; beta ig-h3; kerato-epithelin; RGD-containing collagen-associated protein
• UniProt Protein Name: Transforming growth factor-beta-induced protein ig-h3
• Protein Family: Transforming growth factor-beta-induced protein
• UniProt Gene Name: TGFBI
• UniProt Synonym Gene Names: BIGH3; Beta ig-h3; RGD-CAP
• UniProt Entry Name: BGH3_HUMAN

NCBI Description

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium.

Protein type: Cell adhesion; Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region; plasma membrane; basement membrane; trans-Golgi network

Molecular Function: integrin binding; collagen binding; protein binding; extracellular matrix binding

Biological Process: cell proliferation; extracellular matrix organization and biogenesis; visual perception; response to stimulus; chondrocyte differentiation; negative regulation of cell adhesion; angiogenesis; cell adhesion

Disease: Corneal Dystrophy, Epithelial Basement Membrane; Corneal Dystrophy, Lattice Type Iiia; Corneal Dystrophy Of Bowman Layer, Type Ii; Corneal Dystrophy, Avellino Type; Corneal Dystrophy, Lattice Type I; Corneal Dystrophy Of Bowman Layer, Type I; Corneal Dystrophy, Groenouw Type I