Human ATP Binding Cassette Transporter C10 (ABCC10) ELISA Kit | ABCC10 elisa kit

Human ATP Binding Cassette Transporter C10 (ABCC10) ELISA Kit

Cat. Num. AAA2000199

• Gene Names: ABCC10; MRP7; SIMRP7; EST182763
• Reactivity: Human
• Synonyms: ATP Binding Cassette Transporter C10 (ABCC10); Human ATP Binding Cassette Transporter C10 (ABCC10) ELISA Kit; MRP7; SIMRP7; Multidrug Resistance Associated Protein 7; ABCC10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter C10 (ABCC10).; No significant cross-reactivity or interference between ATP Binding Cassette Transporter C10 (ABCC10) and analogues was observed.
• Sequence Length: 282

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter C10 (ABCC10) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter C10 (ABCC10) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCC10 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C10 (ABCC10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C10 (ABCC10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C10 (ABCC10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter C10 (ABCC10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ABCC10 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 18848267
• NCBI GeneID: 89845
• UniProt Accession #: Q5T3U5; Q8NHX7; Q9H7N2; Q9NXY3; Q9UF48
• Molecular Weight: 158,781 Da
• NCBI Official Full Name: ABCC10 protein
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family C (CFTR/MRP), member 10
• NCBI Official Symbol: ABCC10
• NCBI Official Synonym Symbols: MRP7; SIMRP7; EST182763
• NCBI Protein Information: multidrug resistance-associated protein 7
• UniProt Protein Name: Multidrug resistance-associated protein 7
• Protein Family: ABC transporter C family
• UniProt Gene Name: ABCC10
• UniProt Synonym Gene Names: MRP7; SIMRP7
• UniProt Entry Name: MRP7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This ABC full-transporter is a member of the MRP subfamily which is involved in multi-drug resistance. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

Uniprot Description

ABCC10: ATP-dependent transporter probably involved in cellular detoxification through lipophilic anion extrusion. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: lysosomal membrane; plasma membrane; integral to membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; ATP binding

Biological Process: metabolic process; transmembrane transport