Leptin (Anti-Obesity Protein) (rHuLeptin) Active Protein | lep active protein
Recombinant Human Leptin
Gene Names
lep; ob; xlep
Purity
Greater than 95% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.Purification: Chromatographic
Synonyms
Leptin (Anti-Obesity Protein) (rHuLeptin); Recombinant Human Leptin; Recombinant Human Leptin (Anti-Obesity Protein) (rHuLeptin); lep active protein
Host
E Coli
Specificity
Recombinant Human Leptin (Anti-Obesity Protein) (rHuLeptin)
Purity/Purification
Greater than 95% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Purification: Chromatographic
Purification: Chromatographic
Form/Format
Sterile filtered, lyophilized.
Dimers/Aggregates
<1% by SDSPAGE
Biological Activity
Fully biologically active compared to reference standards. ED50 determined by the leptin dependent stimulation of human OB-R transfected murine BaF3 cells is 0.5-1.6 ng/ml.
Endotoxin Level
<0.1 ng/ug
Protein Content
Determined spectrophotometrically at 280nm (extinction coefficient 0.878) and by RPHPLC analysis against a calibrated standard.
Solubility
Soluble in water and most aqueous buffers above and below the isoelectric point. Dialyzed against 45mM sodium bicarbonate prior to lyophilization.
Stabilizer
None
Preservatives
None
Preparation and Storage
Lyophilized product is stable at room temperature, but should be stored desiccated at -20 degree C. Upon reconstitution, store at 4 degree C. Avoid repeated freeze-thaw cycles.
Related Product Information for lep active protein
Recombinant Human Leptin produced in E. coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids with a molecular mass of 16,240 daltons. This product has full biological activity.
Product Categories/Family for lep active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin
NCBI Official Symbol
lep
NCBI Official Synonym Symbols
ob; xlep
NCBI Protein Information
leptin; obesity homolog
Protein Family
Similar Products
Product Notes
The lep (Catalog #AAA197016) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Leptin (Anti-Obesity Protein) (rHuLeptin), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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