Keratinocyte Growth Factor-2 (rHuKGF-2) Active Protein | FGF10 active protein

Recombinant Human Keratinocyte Growth Factor-2

Cat. Num. AAA197015

• Purity: Greater than 97% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.; Purification: Chromatographic
• Synonyms: Keratinocyte Growth Factor-2 (rHuKGF-2); Recombinant Human Keratinocyte Growth Factor-2; Recombinant Human Keratinocyte Growth Factor-2 (rHuKGF-2); FGF10 active protein

• Host: E Coli
• Specificity: Recombinant Human Keratinocyte Growth Factor-2 (rHuKGF-2)
• Purity/Purification: Greater than 97% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.; Purification: Chromatographic
• Form/Format: Sterile filtered, lyophilized.

• Dimers/Aggregates: <1% by SDSPAGE
• Biological Activity: Fully biologically active compared to reference standards. Specific Activity is 2 x106 Units/mg.
• Endotoxin Level: <0.1 ng/ug
• Protein Content: Determined spectrophotometrically at 280nm (extinction coefficient 1.79) and by RPHPLC analysis against a calibrated standard.
• Solubility: Reconstitute the lyophilized product in sterile water not less than 100 ug/ml. Dialyzed against 5mM sodium phosphate pH 7.4/80 mM NaCl prior to lyophilization.
• Stabilizer: None
• Preservatives: None
• Preparation and Storage: Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20 degree C. Upon reconstitution, store at 4 degree C for 2-7 days or aliquot and store at -20 degree C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.

Related Product Information for FGF10 active protein

Recombinant Human KGF-2 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 170 amino acids with a molecular mass of 19,300 daltons. This product has full biological activity.

Product Categories/Family for FGF10 active protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 2231295
• NCBI GeneID: 2255
• UniProt Accession #: O15520; Q6FHT6; Q96P59; Q6FHR3; Q8NFI9
• Molecular Weight: 23,436 Da
• NCBI Official Full Name: keratinocyte growth factor 2
• NCBI Official Synonym Full Names: fibroblast growth factor 10
• NCBI Official Symbol: FGF10
• NCBI Protein Information: fibroblast growth factor 10; FGF-10; OTTHUMP00000161804; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
• UniProt Protein Name: Fibroblast growth factor 10
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF10
• UniProt Entry Name: FGF10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq]

Uniprot Description

FGF10: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing. Interacts with FGFR1 and FGFR2. Interacts with FGFBP1. Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Cytokine; Cell development/differentiation; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5p13-p12

Cellular Component: extracellular matrix; extracellular space; cell surface; extracellular region; plasma membrane; nucleus

Molecular Function: heparin binding; protein binding; growth factor activity; type 2 fibroblast growth factor receptor binding; chemoattractant activity; fibroblast growth factor receptor binding

Biological Process: nerve growth factor receptor signaling pathway; salivary gland development; somatic stem cell maintenance; urothelial cell proliferation; activation of MAPK activity; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of transcription, DNA-dependent; muscle cell fate commitment; response to lipopolysaccharide; regulation of saliva secretion; embryonic pattern specification; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; positive regulation of fibroblast proliferation; epithelial cell proliferation; positive chemotaxis; embryonic digestive tract morphogenesis; induction of an organ; mesonephros development; embryonic genitalia morphogenesis; spleen development; positive regulation of keratinocyte migration; fibroblast growth factor receptor signaling pathway; positive regulation of DNA repair; positive regulation of urothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; determination of left/right symmetry; metanephros development; positive regulation of epithelial cell proliferation; wound healing; radial glial cell differentiation; positive regulation of mitotic cell cycle; response to estradiol stimulus; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; negative regulation of cell proliferation; establishment of mitotic spindle orientation; positive regulation of MAPKKK cascade; tissue regeneration; positive regulation of lymphocyte proliferation; pancreas development; male genitalia morphogenesis; thyroid gland development; lacrimal gland development; angiogenesis; otic vesicle formation; female genitalia morphogenesis; positive regulation of Notch signaling pathway; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; hair follicle morphogenesis; thymus development; keratinocyte proliferation; regulation of activin receptor signaling pathway; embryonic camera-type eye development; odontogenesis of dentine-containing teeth; limb bud formation; positive regulation of ATPase activity; pituitary gland development; actin cytoskeleton reorganization; white fat cell differentiation; insulin receptor signaling pathway; innate immune response; blood vessel remodeling; positive regulation of Ras protein signal transduction; positive regulation of DNA replication; regulation of smoothened signaling pathway

Disease: Lacrimoauriculodentodigital Syndrome; Aplasia Of Lacrimal And Salivary Glands