Rabbit SAPKalpha (Stress-Activated c-Jun Protein Kinase alpha) Polyclonal Antibody | anti-SAPKalpha antibody

SAPKalpha (Stress-Activated c-Jun Protein Kinase alpha) Polyclonal Antibody

Cat. Num. AAA191466

• Gene Names: Mapk9; SAPK
• Applications: Immunoblot, Western Blot
• Synonyms: SAPKalpha (Stress-Activated c-Jun Protein Kinase alpha); Polyclonal Antibody; SAPKalpha (Stress-Activated c-Jun Protein Kinase alpha) Polyclonal Antibody; SAPK Alpha (JNK2) Antibody; anti-SAPKalpha antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody recognizes human, mouse, and rat SAPKalpha (55kDa).
• Form/Format: Whole rabbit serum.
• Sequence Length: 423

• Applicable Applications for anti-SAPKalpha antibody: Immunoblot
• Application Notes: Immunoblot: 1:500; Dilute in PBS or medium which is identical to that used in the assay system.
• Immunogen: Synthetic peptide corresponding to aa 1-16 of rat SAPKalpha (MSDSKSDGQFYSVQVA (C)).
• Preparation and Storage: This antibody is stable for at least one (1) year at-20 degree C. Avoid multiple freeze-thaw cycles.

Testing Data

(Detection of SAPKalpha in mouse brain tissue extract with SAPKalpha antibody diluted 1:500.)

Related Product Information for anti-SAPKalpha antibody

The stress-activated protein kinases alpha and gamma (SAPK alpha and gamma), also known as cJun NH2-terminal kinases 1 and 2 (Jnk1/2) are serine/ threonine protein kinases belonging to the MAP kinase family. Extracellular stimuli leading to cell stress include inflammatory cytokines (via Rho and Rac), growth factors, protein synthesis inhibitors and osmotic stress. Both Jnk1 and Jnk2 must be dually phosphorylated in the activation loop, at threonine 183 and tyrosine 185, to achieve full enzymatic activity.

NCBI and Uniprot Product Information

• NCBI GI #: 395759202
• NCBI GeneID: 50658
• NCBI Accession #: NP_001257473.1
• NCBI GenBank Nucleotide #: NM_001270544.1
• UniProt Accession #: P49186
• Molecular Weight: 48,107 Da
• NCBI Official Full Name: mitogen-activated protein kinase 9 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 9
• NCBI Official Symbol: Mapk9
• NCBI Official Synonym Symbols: SAPK
• NCBI Protein Information: mitogen-activated protein kinase 9
• UniProt Protein Name: Mitogen-activated protein kinase 9
• UniProt Gene Name: Mapk9
• UniProt Synonym Gene Names: Jnk2; Prkm9; MAP kinase 9; MAPK 9

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases are multifunctional proteins. They are involved in a wide variety of cellular processes such as growth, proliferation, differentiation, transcription regulation, and development. They function in stress responses, apoptosis, inflammation and transformation. For example, this kinase plays a dominant role in mediating proliferation of lung cancer and prostate cancer cells, and in regulation of osmotic stress-induced tight junction disruption. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2012]

Uniprot Description

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation.