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Testing Data (Detection of choline acetyltransferase in mouse brain lysate diluted 1:1,000.)

Rabbit Choline acetyltransferase Polyclonal Antibody | anti-CHAT antibody

Choline Acetyltransferase Polyclonal Antibody

Gene Names
CHAT; CMS6; CMS1A; CMS1A2; CHOACTASE
Applications
Immunoblot, Western Blot, Immunofluorescence
Synonyms
Choline acetyltransferase; Polyclonal Antibody; Choline Acetyltransferase Polyclonal Antibody; anti-CHAT antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Specificity
This antibody recognizes human and mouse choline acetyltransferase
Form/Format
100ug (1mg/ml) peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Sequence Length
630
Applicable Applications for anti-CHAT antibody
Immunoblot, Immunofluorescence (IF)
Application Notes
Immunoblot: 1:1,000. A band of ~80kDa is detected.
IF: 1:100
Immunogen
Synthetic peptide corresponding to amino acids from the N-terminus to the mid-protein of human choline o-acetyltransferase.

Testing Data

(Detection of choline acetyltransferase in mouse brain lysate diluted 1:1,000.)

Testing Data (Detection of choline acetyltransferase in mouse brain lysate diluted 1:1,000.)

Testing Data

(Detection of choline acetyltransferase in human neuroblastoma cell line SK-N-BE diluted 1:100. DAPI (blue) nuclear stain, Texas red actin stain, and FITC (green) choline acetyltransferase stain.)

Testing Data (Detection of choline acetyltransferase in human neuroblastoma cell line SK-N-BE diluted 1:100. DAPI (blue) nuclear stain, Texas red actin stain, and FITC (green) choline acetyltransferase stain.)
Related Product Information for anti-CHAT antibody
Choline acetyltransferase (ChAT) catalyzes the transfer of an acetyl group from acetyl-CoA to choline to produce acetylcholine. ChAT is found in high concentration in the central nervous system and peripheral nervous system. Alterations in concentrations of acetylcholine and ChAT have been associated with Alzheimer disease and Amyotrophic Lateral Sclerosis (ALS).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74,361 Da
NCBI Official Full Name
choline O-acetyltransferase isoform 1
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
CHAT
NCBI Official Synonym Symbols
CMS6; CMS1A; CMS1A2; CHOACTASE
NCBI Protein Information
choline O-acetyltransferase
UniProt Protein Name
Choline O-acetyltransferase
UniProt Gene Name
CHAT
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase

NCBI Description

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

Uniprot Description

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Research Articles on CHAT

Similar Products

Product Notes

The CHAT chat (Catalog #AAA191355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Choline acetyltransferase can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunofluorescence (IF). Immunoblot: 1:1,000. A band of ~80kDa is detected. IF: 1:100. Researchers should empirically determine the suitability of the CHAT chat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Choline acetyltransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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