Bcl-2 Polyclonal Antibody | anti-Bcl-2 antibody

Bcl-2 (Phospho-Thr56)

Cat. Num. AAA191075

• Gene Names: BCL2; Bcl-2; PPP1R50
• Applications: Immunohistochemistry
• Synonyms: Bcl-2; Polyclonal Antibody; Bcl-2 (Phospho-Thr56); anti-Bcl-2 antibody

• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human Bcl-2 only when phosphorylated at threonine 56.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 239

• Applicable Applications for anti-Bcl-2 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: use at dilution of 1:50-1:100.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation site of threonine 56 (G-H-T(p)-P-H) derived from human Bcl-2 and conjugated to KLH.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term.

Immunohistochemistry (IHC)

Related Product Information for anti-Bcl-2 antibody

Bcl-2 protein suppresses apoptosis in a variety of cellular systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspase. Bcl-2 inhibits caspase acativity either by preventing release of cytochrome c from the mitochondria and/or by binding to apoptosis-activating factor (Apaf-1). The predicted molecular weight of Bcl-2 is ~26kDa.

NCBI and Uniprot Product Information

• NCBI GI #: 72198189
• NCBI GeneID: 596
• NCBI Accession #: NP_000624.2
• NCBI GenBank Nucleotide #: NM_000633.2
• UniProt Accession #: P10415; P10416; Q13842; Q16197; C9JHD5
• Molecular Weight: 22,337 Da
• NCBI Official Full Name: apoptosis regulator Bcl-2 alpha isoform
• NCBI Official Synonym Full Names: BCL2, apoptosis regulator
• NCBI Official Symbol: BCL2
• NCBI Official Synonym Symbols: Bcl-2; PPP1R50
• NCBI Protein Information: apoptosis regulator Bcl-2
• UniProt Protein Name: Apoptosis regulator Bcl-2
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2

NCBI Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions.

Protein type: Apoptosis; Autophagy; Membrane protein, integral; Oncoprotein

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: cytoplasm; endoplasmic reticulum; membrane; mitochondrial outer membrane; mitochondrion; nuclear membrane; nucleus; pore complex

Molecular Function: BH3 domain binding; channel activity; channel inhibitor activity; identical protein binding; protease binding; protein binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; ubiquitin protein ligase binding

Biological Process: apoptosis; B cell proliferation; B cell receptor signaling pathway; defense response to virus; DNA damage response, signal transduction resulting in induction of apoptosis; endoplasmic reticulum calcium ion homeostasis; humoral immune response; induction of apoptosis via death domain receptors; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of cellular pH reduction; negative regulation of mitochondrial depolarization; negative regulation of neuron apoptosis; neuron apoptosis; positive regulation of B cell proliferation; positive regulation of cell growth; protein polyubiquitination; regulation of calcium ion transport; regulation of mitochondrial membrane permeability; regulation of mitochondrial membrane potential; regulation of protein heterodimerization activity; regulation of protein homodimerization activity; regulation of transmembrane transporter activity; release of cytochrome c from mitochondria; response to cytokine stimulus; response to DNA damage stimulus; response to drug; response to iron ion; response to nicotine; response to toxin