Mouse CD74 Monoclonal Antibody | anti-CD74 antibody

Anti-CD74 Monoclonal Antibody

Cat. Num. AAA190488

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Applications: Flow Cytometry, Immunoblot, Western Blot, Immunocytochemistry, Immunoprecipitation
• Synonyms: CD74; Monoclonal Antibody; Anti-CD74 Monoclonal Antibody; anti-CD74 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: CD74 [Mouse monoclonal antibody recognizes human CD74.]; This antibody recognizes human CD74.
• Form/Format: 100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.

• Applicable Applications for anti-CD74 antibody: Flow Cytometry, Immunoblot, Immunocytochemistry, Immunoprecipitation
• Application Notes: Immunoblotting: use at 1ug/ml. A doublet band of ~33-35 kDa is detected.; Immunoprecipitation: use at 12ug/ml.; Immunocytochemistry: working concentration is sample dependent.; Flow cytometry: working concentration is sample dependent. These are recommended concentrations. User should determine optimal concentrations for their application.; Positive control: PALA cell lysate.
• Antigen: Synthetic peptide corresponding to the human CD74 invariant chain (aa 12-28) conjugated to KLH.
• Preservatives: 0.09% sodium azide
• Other Additives: 50% glycerol
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Related Product Information for anti-CD74 antibody

CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain. CD74 functions mainly as an MHC class II chaperone which promotes ER exit of MHC class II molecules by directing them to endocytic compartments, preventing peptide binding in the ER, and contributing to peptide "editing" in the MHC class II compartment. CD74 has recently been shown to have a role as an accessory signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF).

Product Categories/Family for anti-CD74 antibody

Neuroscience and Signal Transduction Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 119582135
• NCBI GeneID: 972
• UniProt Accession #: P04233; Q14597; Q29832; Q5U0J8; Q8SNA0; Q8WLP6; A8K7R1; B4DNE8; D3DQG3; D3DQG4; O19685; O78208
• Molecular Weight: 33,516 Da
• NCBI Official Full Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), isoform CRA_c
• NCBI Official Synonym Full Names: CD74 molecule, major histocompatibility complex, class II invariant chain
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain; p33; HLA-DR-gamma; OTTHUMP00000224283; OTTHUMP00000224284; OTTHUMP00000224285; OTTHUMP00000224286; OTTHUMP00000224288; OTTHUMP00000224338; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• Protein Family: H-2 class II histocompatibility antigen
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG
• UniProt Entry Name: HG2A_HUMAN

NCBI Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CD74: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74- ROS1 chimeric protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: lysosomal lumen; cell surface; lysosomal membrane; integral to membrane; MHC class II protein complex; Golgi membrane; multivesicular body; membrane; plasma membrane; vacuole; trans-Golgi network membrane; intracellular; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; cytokine binding; beta-amyloid binding; MHC class II protein binding, via antigen binding groove; nitric-oxide synthase binding; MHC class II protein binding

Biological Process: activation of MAPK activity; positive regulation of dendritic cell antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class II; defense response; prostaglandin biosynthetic process; signal transduction; negative regulation of T cell differentiation; intracellular protein transport; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of fibroblast proliferation; negative regulation of mature B cell apoptosis; positive regulation of B cell proliferation; protein complex assembly; positive thymic T cell selection; negative regulation of peptide secretion; positive regulation of T-helper 2 type immune response; chaperone cofactor-dependent protein folding; regulation of macrophage activation; T cell selection; negative thymic T cell selection; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell differentiation; immunoglobulin mediated immune response; antigen processing and presentation of endogenous antigen; positive regulation of cytokine and chemokine mediated signaling pathway; negative regulation of apoptosis