Mouse IGF-1 receptor Monoclonal Antibody | anti-IGF1R antibody

IGF-1 Receptor Monoclonal Antibody

Cat. Num. AAA190274

• Gene Names: IGF1R; IGF1R
• Applications: Immunoblot, Western Blot, Immunoprecipitation
• Synonyms: IGF-1 receptor; Monoclonal Antibody; IGF-1 Receptor Monoclonal Antibody; Anti-IGF-1 receptor; IGF-1 Receptor; anti-IGF1R antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Anti-IGF-1 receptor [Murine MAb recognizes human IGF-1 receptor; stimulates binding of IGF-1 and 2, stimulates cell growth.]; All three clones are specific for the human IGF-1 receptor; This product (3B7) stimulates binding of IGF-1 and -2; stimulates basal and IGF- 1 stimulated cell growth.
• Form/Format: 100 ug Protein G-purified antibody in PBS, pH 7.4
• Sequence Length: 304

• Applicable Applications for anti-IGF1R antibody: Immunoblot, Immunoprecipitation
• Application Notes: Use these antibodies in research studies to delineate signal transduction pathways specific for IGF's and for detecting IGF-1 receptors in crude samples. 35101 may be used to immunoprecipitate IGF-1 receptors. 35102 and 35103 can recognize the IGF-1 alpha subunit on immunoblots.
• Antigen: Purified human placental IGF-1 receptors.
• Stabilizer: None
• Preservatives: None. Available on request.
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: These antibodies are stable for at least one (1) year at -20 degree C to -70 degree C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles

Product Categories/Family for anti-IGF1R antibody

Growth Factors/Cytokines/Receptor Antibodies

References

Xiong et al. (1992) Proc Natl Acad Sci USA 89: 5356; Li et al. (1993) Biochem Biophys Res Commun 196: 92.

NCBI and Uniprot Product Information

• NCBI GI #: 47523430
• NCBI GeneID: 397350
• NCBI Accession #: NP_999337.1
• NCBI GenBank Nucleotide #: NM_214172.1
• UniProt Accession #: Q29000; Q28951
• Molecular Weight: 34,615 Da
• NCBI Official Full Name: insulin-like growth factor 1 receptor
• NCBI Official Symbol: IGF1R
• NCBI Official Synonym Symbols: IGF1R
• NCBI Protein Information: insulin-like growth factor 1 receptor; IGF-1 receptor; IGF-I receptor; insulin-like growth factor I receptor; insulin-like growth factor receptor 1; insulin-like growth factor-I receptor; insulin-like growth factor 1 receptor beta subunit
• UniProt Protein Name: Insulin-like growth factor 1 receptor
• Protein Family: Insulin-like growth factor 1 receptor
• UniProt Gene Name: IGF1R
• UniProt Entry Name: IGF1R_PIG

Uniprot Description

Function: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Cofactor: Manganese

Subunit structure: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Forms a hybrid receptor with INSR, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with GNB2L1/RACK1

Subcellular location: Membrane; Single-pass type I membrane protein.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.Contains 1 fibronectin type-III domain.Contains 1 protein kinase domain.