Mouse FGF Receptor 1 Monoclonal Antibody | anti-FGFR1 antibody

FGF Receptor 1 Monoclonal Antibody

Cat. Num. AAA190161

• Gene Names: FGFR1; CEK; FLG; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; FLJ99988; bFGF-R-1
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Synonyms: FGF Receptor 1; Monoclonal Antibody; FGF Receptor 1 Monoclonal Antibody; Anti-FGF Receptor 1; anti-FGFR1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Anti-FGF Receptor 1 [Murine MAb recognizes a and b isoforms; epitope unknown within a sequence of amino acids 179-195 in Ig Loop II.]; Reacts with both alpha and beta isoforms. Epitope is within the sequence pro179 to lys195 in Ig loop II.
• Form/Format: 250 ug Protein G-purified antibody in PBS, pH 7.4

• Applicable Applications for anti-FGFR1 antibody: Western Blot, Immunohistochemistry, Immunoprecipitation
• Application Notes: Western Blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculoviral, or native FGFr1.; Immunoprecipitation: native, recombinant, and ligand-labeled FGFr1. Immunohistochemistry: under investigation.
• Antigen: Recombinant human ectodomain of FGFr1 beta expressed in E. coli from pro23 to his415; antigen contained NH2-terminal gly-ser-pro-glyile and COOH-terminal glu-phe sequences.
• Stabilizer: None
• Preservatives: None. Available on request.
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: These antibodies are stable for at least one (1) year at -20 degree C to -70 degree C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles

Product Categories/Family for anti-FGFR1 antibody

Growth Factors/Cytokines/Receptor Antibodies

References

Xu et al. (1992) J Biol Chem 267: 1779; Robinson et al. (1995) Development 121: 3959.

NCBI and Uniprot Product Information

• NCBI GI #: 182530
• NCBI GeneID: 2260
• UniProt Accession #: P11362; P17049; Q02063; Q02065; Q14306; Q14307; Q53H63; Q5BJG2; Q8N685; A8K6T9; A8K8V5; C1KBH8; Q53G05; Q59H40; Q7Z2S2
• Molecular Weight: 91,868 Da
• NCBI Official Full Name: FGF receptor-1
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 1
• NCBI Official Symbol: FGFR1
• NCBI Official Synonym Symbols: CEK; FLG; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; FLJ99988; bFGF-R-1
• NCBI Protein Information: basic fibroblast growth factor receptor 1; OTTHUMP00000190874; OTTHUMP00000190875; OTTHUMP00000190876; OTTHUMP00000190877; OTTHUMP00000190879; OTTHUMP00000190880; OTTHUMP00000190881; OTTHUMP00000190885; proto-oncogene c-Fgr; FMS-like tyrosine kinase 2; hydroxyaryl-protein kinase; fms-related tyrosine kinase 2; heparin-binding growth factor receptor
• UniProt Protein Name: Basic fibroblast growth factor receptor 1
• Protein Family: Fibroblast growth factor receptor
• UniProt Gene Name: FGFR1
• UniProt Synonym Gene Names: FGFBR; FLG; FLT2
• UniProt Entry Name: FGFR1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]

Uniprot Description

FGFR1: a receptor tyrosine kinase of the highly-conserved fibroblast growth factor receptor (FGFR). Binds both acidic and basic fibroblast growth factors and is involved in limb induction. Point mutations cause Pfeffer syndrome (finger and toe malformations and other skeletal errors) and dominant Kallmann syndrome 2. Stem cell leukemia lymphoma syndrome (SCLL) may be caused by a t(8;13)(p12;q12) translocation that fuses a zinc finger gene, ZNF198, to FGFR1. Various myeloproliferative disorders have been linked to translocations that fuse FGFR1 to FOP, FIM, CEP1 or the atypical kinase, Bcr. Inhibitor: SU5402. 20 isoforms of the human protein produced by alternative splicing have been described.

Protein type: Kinase, protein; Oncoprotein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; TK group; FGFR family

Chromosomal Location of Human Ortholog: 8p11.23-p11.22

Cellular Component: integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; integral to membrane; plasma membrane; cytosol; nucleus; receptor complex

Molecular Function: heparin binding; identical protein binding; protein binding; protein homodimerization activity; fibroblast growth factor binding; fibroblast growth factor receptor activity; protein-tyrosine kinase activity; ATP binding

Biological Process: paraxial mesoderm development; axon guidance; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; cell maturation; neuron migration; middle ear morphogenesis; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; positive regulation of MAP kinase activity; sensory perception of sound; positive regulation of MAPKKK cascade; ureteric bud development; induction of an organ; regulation of cell differentiation; midbrain development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; chondrocyte differentiation; angiogenesis; skeletal development; positive regulation of cardiac muscle cell proliferation; embryonic limb morphogenesis; epidermal growth factor receptor signaling pathway; inner ear morphogenesis; cell migration; chordate embryonic development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; in utero embryonic development; outer ear morphogenesis; MAPKKK cascade; positive regulation of cell cycle; neuroblast division in the ventricular zone; positive regulation of phosphoinositide 3-kinase cascade; mesenchymal cell differentiation; skeletal morphogenesis; insulin receptor signaling pathway; auditory receptor cell development; innate immune response; positive regulation of neuron differentiation; regulation of lateral mesodermal cell fate specification

Disease: Pfeiffer Syndrome; Hypogonadotropic Hypogonadism 2 With Or Without Anosmia; Jackson-weiss Syndrome; Trigonocephaly 1; Osteoglophonic Dysplasia