Amyloid Precursor, alpha form, APPalpha Recombinant Protein | APPalpha recombinant protein

Recombinant Human Amyloid Precursor Protein, alpha form, APPalpha

Cat. Num. AAA184016

• Gene Names: APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• Applications: Western Blot
• Purity: Affinity chromatography. >95% as estimated by SDS-PAGE stained with Instant Blue Stain (Expedeon).
• Synonyms: Amyloid Precursor; alpha form; APPalpha; Recombinant Human Amyloid Precursor Protein; Amyloid-beta A4 protein; ABPP; APPI; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta precursor protein; Cerebral vascular amyloid peptide (CVAP); PreA4; Protease nexin-II (PN-II); Soluble APP-alpha (S-APP-alpha); Isoform APP695; APPalpha recombinant protein

• Host: HEK293 Cells
• Purity/Purification: Affinity chromatography. >95% as estimated by SDS-PAGE stained with Instant Blue Stain (Expedeon).
• Form/Format: Liquid. PBS 20% Glycerol
• Sequence Positions: 18-612aa
• Sequence: HHHHHHHHHHGSGLEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVRVPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQK

• Applicable Applications for APPalpha recombinant protein: Western Blot (WB), Immunoassay (IA), Functional Studies
• Source: Human
• Tag: 10xHis at the N-terminus
• Endotoxin: Less than 0.1ng/ug (1 IEU/ug), as measured by LAL method.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store at -20 degree or -70 degree C for at least 12 months; Shipping: Dry ice or frozen gel packs

SDS-Page

Related Product Information for APPalpha recombinant protein

Functions as a cell surface receptor and performs physiological functions on the surface ofneurons relevant to neurite growth, neuronal adhesion and axonogenesis (1). Involved incell mobility and transcription regulation through protein-protein interactions. Canpromote transcription activation through binding to APBB1-KAT5 and inhibits Notchsignalling through interaction with Numb. Couples to apoptosis-inducing pathways such asthose mediated by G(O) and JIP (2). Inhibits G(O) alpha ATPase activity. Acts as a kinesin Imembrane receptor, mediating the axonal transport of beta-secretase and presenilin 1.Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro,copper-metallated APP induces neuronal death directly or is potentiated through Cu2+-mediated low-density lipoprotein oxidation (3). Can regulate neurite outgrowth throughbinding to components of the extracellular matrix such as heparin and collagen I and IV. APP is involved in Alzheimer disease which is caused by mutations affecting the gene codingfor the protein (4). Alzheimer disease is a neurodegenerative disorder characterized byprogressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteinsas intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascularamyloid deposits. The major constituents of these plaques are neurotoxic amyloid-betaprotein 40 and amyloid-beta protein 42, that are produced by the proteolysis of the transmembrane APP protein (5).

References

1. Kang et al. (1987) Nature, 733-736
2. Goldstein. (2012) Prog. Neurobiol., 186-190
3. Acevedo et al. (2011) J. Biol. Chem., 8252-8262
4. Citron et al. (1995) Neuron, 661-670
5. Kant and Goldstein. (2015) Dev. Cell, 502-515

NCBI and Uniprot Product Information

• NCBI GI #: 4502167
• NCBI GeneID: 351
• NCBI Accession #: NP_000475.1
• NCBI GenBank Nucleotide #: NM_000484.3
• UniProt Accession #: P05067; P09000; P78438; Q13764; Q13778; Q13793; Q16011; B2R5V1; B4DII8; D3DSD1; D3DSD2; D3DSD3
• Molecular Weight: 86,943 Da
• NCBI Official Full Name: amyloid beta A4 protein isoform a
• NCBI Official Synonym Full Names: amyloid beta (A4) precursor protein
• NCBI Official Symbol: APP
• NCBI Official Synonym Symbols: AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• NCBI Protein Information: amyloid beta A4 protein; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; alzheimer disease amyloid protein; cerebral vascular amyloid peptide
• UniProt Protein Name: Amyloid beta A4 protein
• UniProt Gene Name: APP
• UniProt Synonym Gene Names: A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)
• UniProt Entry Name: A4_HUMAN

NCBI Description

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

APP: a cell surface receptor that influences neurite growth, neuronal adhesion and axonogenesis. Cleaved by secretases to form a number of peptides, some of which bind to the acetyltransferase complex Fe65/TIP60 to promote transcriptional activation. The Abeta peptide is released from the cell, its extracellular deposition and accumulation form the main components of amyloid plaques in Alzheimer's disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis. Can promote transcription activation through binding to Fe65-Tip60 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(O) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. Induces a RAGE-dependent pathway that activates p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, JIP1, SHC1 and, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Amyloid beta-42 binds nAChRA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. 10 isoforms of the human protein are produced by alternative splicing. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Belongs to the APP family.

Protein type: Cell surface; Receptor, misc.; Transcription factor; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; extracellular region; integral to membrane; dendritic spine; coated pit; intercellular junction; cytosol; ER to Golgi transport vesicle; lipid raft; ciliary rootlet; nuclear envelope lumen; perinuclear region of cytoplasm; axon; apical part of cell; cytoplasm; plasma membrane; synapse; spindle midzone; dendritic shaft; neuromuscular junction; endosome; receptor complex

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; identical protein binding; protein binding; protease activator activity; enzyme binding; DNA binding; transition metal ion binding; PTB domain binding; acetylcholine receptor binding; receptor binding

Biological Process: extracellular matrix organization and biogenesis; adult locomotory behavior; locomotory behavior; mRNA polyadenylation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of translation; platelet degranulation; synaptic growth at neuromuscular junction; forebrain development; dendrite development; visual learning; collateral sprouting in the absence of injury; neuromuscular process controlling balance; cell adhesion; neurite development; cholesterol metabolic process; platelet activation; Notch signaling pathway; cellular copper ion homeostasis; regulation of epidermal growth factor receptor activity; axon cargo transport; mating behavior; regulation of multicellular organism growth; endocytosis; axon midline choice point recognition; smooth endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; negative regulation of neuron differentiation; axonogenesis; suckling behavior; ionotropic glutamate receptor signaling pathway; regulation of synapse structure and activity; regulation of protein binding; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; blood coagulation; neuron remodeling

Disease: Alzheimer Disease