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Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD177 (MEM-166) APC.)

Mouse CD177 Antibody | anti-CD177 antibody

Anti-Hu CD177 APC

Gene Names
CD177; NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP
Reactivity
Non-human primates, Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD177; Anti-Hu CD177 APC; CD177 molecule; NB1; PRV1; HNA2A; anti-CD177 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Non-human primates, Human
Isotype
IgG1
Clone Number
MEM-166
Specificity
The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
Purity/Purification
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Sequence Length
437
Applicable Applications for anti-CD177 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
Human granulocytes
Conjugation
APC
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD177 (MEM-166) APC.)

Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD177 (MEM-166) APC.)
Related Product Information for anti-CD177 antibody
CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
CD177 antigen
NCBI Official Synonym Full Names
CD177 molecule
NCBI Official Symbol
CD177
NCBI Official Synonym Symbols
NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP
NCBI Protein Information
CD177 antigen
UniProt Protein Name
CD177 antigen
Protein Family
UniProt Gene Name
CD177
UniProt Synonym Gene Names
NB1; PRV1; HNA-2a; NB1 GP; PRV-1
UniProt Entry Name
CD177_HUMAN

NCBI Description

This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014]

Uniprot Description

CD177: Induced by CSF3 in resting granulocytes. Induced in patients with polycythemia vera (PV) and with essential thrombocythemia (ET). 3 isoforms of the human protein are produced by alternative splicing

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: plasma membrane

Biological Process: blood coagulation; leukocyte migration

Research Articles on CD177

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Product Notes

The CD177 cd177 (Catalog #AAA1801065) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD177 APC reacts with Non-human primates, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD177 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD177 cd177 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD177, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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