Rat anti-Mouse CD4 Monoclonal Antibody | anti-CD4 antibody

Anti-Ms CD4 PE

Cat. Num. AAA1800655

• Gene Names: Cd4; L3T4; Ly-4
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD4; Monoclonal Antibody; Anti-Ms CD4 PE; CD4 molecule; T4/Leu-3; L3T4; anti-CD4 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: GK1.5
• Specificity: The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 457

• Applicable Applications for anti-CD4 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: Mouse CTL clone V4 cells
• Conjugation: PE
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Separation of murine CD4 positive cells (red-filled) from murine CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) PE antibody (concentration in sample 0,5 ug/ml).)

Related Product Information for anti-CD4 antibody

CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

NCBI and Uniprot Product Information

• NCBI GI #: 7304953
• NCBI GeneID: 12504
• NCBI Accession #: NP_038516.1
• NCBI GenBank Nucleotide #: NP_038516.1
• UniProt Accession #: P06332
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Synonym Full Names: CD4 antigen
• NCBI Official Symbol: Cd4
• NCBI Official Synonym Symbols: L3T4; Ly-4
• NCBI Protein Information: T-cell surface glycoprotein CD4
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: Cd4
• UniProt Entry Name: CD4_MOUSE