Typical Testing Data/Standard Curve (for reference only)
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Human A1BG/alpha 1B-Glycoprotein ELISA Kit | A1BG elisa kit
Human A1BG/alpha 1B-Glycoprotein PicoKine ELISA Kit
Gene Names
A1BG; A1B; ABG; GAB; HYST2477
Reactivity
Human
Synonyms
A1BG/alpha 1B-Glycoprotein; Human A1BG/alpha 1B-Glycoprotein PicoKine ELISA Kit; A1B; A1BG; ABG; GAB; HYST2477; P04217; Alpha-1B-glycoprotein; A1BG elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human A1BG
Sequence Length
495
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for A1BG elisa kit
Principle of the Assay: The Picokine™ Human A1BG Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human A1BG with a 96-well strip plate that is pre-coated with antibody specific for A1BG. The detection antibody is a biotinylated antibody specific for A1BG. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human A1BG with immunogen: Expression system for standard: NS0; Immunogen sequence: A22-S495. The kit is analytically validated with ready-to-use reagents. To measure Human A1BG, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human A1BG in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human A1BG in the sample.
Background/Introduction: Alpha-1-B glycoprotein is a 54.3 kDa protein in humans that is encoded by the A1BG gene. The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. Patients who have pancreatic ductal adenocarcinoma show an overexpression of A1BG in pancreatic juice.
Background/Introduction: Alpha-1-B glycoprotein is a 54.3 kDa protein in humans that is encoded by the A1BG gene. The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. Patients who have pancreatic ductal adenocarcinoma show an overexpression of A1BG in pancreatic juice.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,718 Da
NCBI Official Full Name
alpha-1B-glycoprotein
NCBI Official Synonym Full Names
alpha-1-B glycoprotein
NCBI Official Symbol
A1BG
NCBI Official Synonym Symbols
A1B; ABG; GAB; HYST2477
NCBI Protein Information
alpha-1B-glycoprotein
UniProt Protein Name
Alpha-1B-glycoprotein
Protein Family
UniProt Gene Name
A1BG
NCBI Description
The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. [provided by RefSeq, Jul 2008]
Uniprot Description
A1BG: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19q13.43
Cellular Component: extracellular region; extracellular space
Biological Process: platelet degranulation
Research Articles on A1BG
Similar Products
Product Notes
The Human A1BG a1bg (Catalog #AAA178733) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178733 ELISA Kit recognizes Human A1BG. It is sometimes possible for the material contained within the vial of "A1BG/alpha 1B-Glycoprotein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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