Vitronectin Polyclonal Antibody | anti-VTN antibody

Anti-Vitronectin Antibody

Cat. Num. AAA178298

• Gene Names: VTN; VN; V75; VNT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Vitronectin; Polyclonal Antibody; Anti-Vitronectin Antibody; Complement S Protein; Epibolin; S Protein; S-protein; Serum Spreading Factor; Serum-spreading factor; Somatomedin B; Somatomedin-B; V75; Vitronectin V10 subunit; Vitronectin V65 subunit; VN; VNT; VTN; VTNC_HUMAN; vitronectin; anti-VTN antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 478

• Applicable Applications for anti-VTN antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Vitronectin (446-472 aa RVNLRTRRVDTVDPPYPRSIAQYWLGC), different from the related mouse sequence by two amino acids.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Vitronectin Picoband antibody, MBS178298, Western blottingAll lanes: Anti Vitronectin (MBS178298) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: HEPG2 Whole Cell Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ugLane 5: HEPA Whole Cell Lysate at 40ugPredicted bind size: 54KDObserved bind size: 54KD )

Immunohistochemistry (IHC)

(Anti- Vitronectin Picoband antibody, MBS178298, IHC(P)IHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-VTN antibody

Description: Rabbit IgG polyclonal antibody for Vitronectin(VTN) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Vitronectin, also known as VTN, is a protein that in humans is encoded by the VTN gene. The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Also vitronectin serves to regulate proteolysis initiated by plasminogen activation. In addition, vitronectin is a component of platelets and is, thus, involved in hemostasis.

References

1. "Entrez Gene: M Vitronectin". 2. Jenne D, Stanley KK (October 1987). "Nucleotide sequence and organization of the human S-protein gene: repeating peptide motifs in the "pexin" family and a model for their evolution". Biochemistry 26 (21): 6735-42. 3. Preissner KT, Seiffert D (January 1998)."Role of vitronectin and its receptors in haemostasis and vascular remodeling" (– SCHOLAR SEARCH). Thromb. Res. 89(1): 1-21.

NCBI and Uniprot Product Information

• NCBI GI #: 88853069
• NCBI GeneID: 7448
• NCBI Accession #: NP_000629.3
• NCBI GenBank Nucleotide #: NM_000638.3
• UniProt Accession #: P04004; P01141; Q9BSH7; B2R7G0
• Molecular Weight: 54,306 Da
• NCBI Official Full Name: vitronectin
• NCBI Official Synonym Full Names: vitronectin
• NCBI Official Symbol: VTN
• NCBI Official Synonym Symbols: VN; V75; VNT
• NCBI Protein Information: vitronectin
• UniProt Protein Name: Vitronectin
• Protein Family: Vitronectin
• UniProt Gene Name: VTN
• UniProt Synonym Gene Names: VN
• UniProt Entry Name: VTNC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]

Uniprot Description

VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

Protein type: Cell adhesion; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: extracellular matrix binding; heparin binding; integrin binding; polysaccharide binding; protein binding; scavenger receptor activity

Biological Process: cell adhesion; cell adhesion mediated by integrin; cell-matrix adhesion; extracellular matrix organization and biogenesis; immune response; negative regulation of blood coagulation; oligodendrocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein binding; positive regulation of receptor-mediated endocytosis; positive regulation of smooth muscle cell migration; positive regulation of vascular endothelial growth factor receptor signaling pathway; receptor-mediated endocytosis; regulation of complement activation