anti-Human, Rat Rad51 Polyclonal Antibody | anti-RAD51 antibody

Anti-Rad51 Antibody

Cat. Num. AAA178102

• Gene Names: RAD51; RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Rad51; Polyclonal Antibody; Anti-Rad51 Antibody; DNA repair protein RAD51 homolog 1; BRCA1/BRCA2 containing complex; subunit 5; BRCC 5; BRCC5; DNA repair protein rhp51; E coli RecA homolog; Homolog of E coli RecA; homolog of S cerevisiae RAD51; HRAD51; HsRad51; HsT16930; MRMV2; Rad 51; RAD51; RAD51 homolog (RecA homolog; E. coli) (S. cerevisiae); RAD51 homolog A; RAD51 homolog; RAD51 homolog S. cerevisiae; RAD51 recombinase; RAD51 S cerevisiae homolog; S. cerevisiae; homolog of; RAD51_HUMAN; RAD51A; RECA; RecA homolog E. coli; RecA like protein; RecA; E. coli; recombination protein A; anti-RAD51 antibody

• Reactivity: Human, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 340

• Applicable Applications for anti-RAD51 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human Rad51 recombinant protein (Position: M1-E258). Human Rad51 shares 98% amino acid (aa) sequence identity with mouse Rad51.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Rad51 Picoband antibody, MBS178102, Western blottingAll lanes: Anti Rad51 (MBS178102) at 0.5ug/mlLane 1: 22RV1 Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugLane 3: PANC Whole Cell Lysate at 40ugLane 4: U87 Whole Cell Lysate at 40ugLane 5: CEM Whole Cell Lysate at 40ugLane 6: MM231 Whole Cell Lysate at 40ugPredicted bind size: 37KDObserved bind size: 37KD )

Immunohistochemistry (IHC)

(Anti- Rad51 Picoband antibody, MBS178102, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti- Rad51 Picoband antibody, MBS178102, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Western Blot (WB)

(Applications: Western BlotSample: MEFsDetection: LICOR Odyssey"The goal was to confirm the knockdown of mouse Rad51 via siRNA. Confirmed siRNA knockdown expression. Good detection. Recommended.")

Related Product Information for anti-RAD51 antibody

Description: Rabbit IgG polyclonal antibody for DNA repair protein RAD51 homolog 1(RAD51) detection. Tested with WB, IHC-P in Human;Rat.

Background: DNA repair protein RAD51 homolog 1, also known as RAD51A, is a human gene. The Rad51 gene, HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair. BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. RAD51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.

References

1. Kato, M.; Yano, K.; Matsuo, F.; Saito, H.; Katagiri, T.; Kurumizaka, H.; Yoshimoto, M.; Kasumi, F.; Akiyama, F.; Sakamoto, G.; Nagawa, H.; Nakamura, Y.; Miki, Y. : Identification of Rad51 alteration in patients with bilateral breast cancer. J. Hum. Genet. 45: 133-137, 2000. 2. Daniel DC (2002). "Highlight: BRCA1 and BRCA2 proteins in breast cancer.". Microsc. Res. Tech. 59 (1): 68-83.

NCBI and Uniprot Product Information

• NCBI GI #: 256017143
• NCBI GeneID: 5888
• NCBI Accession #: NP_001157741.1
• NCBI GenBank Nucleotide #: NM_001164269.1
• UniProt Accession #: Q06609; Q6FHX9; Q6ZNA8; Q9BV60; B0FXP0; B2R8T6
• Molecular Weight: 36,780 Da
• NCBI Official Full Name: DNA repair protein RAD51 homolog 1 isoform 2
• NCBI Official Synonym Full Names: RAD51 recombinase
• NCBI Official Symbol: RAD51
• NCBI Official Synonym Symbols: RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• NCBI Protein Information: DNA repair protein RAD51 homolog 1
• UniProt Protein Name: DNA repair protein RAD51 homolog 1
• Protein Family: RAD51-like protein
• UniProt Gene Name: RAD51
• UniProt Synonym Gene Names: RAD51A; RECA; HsRAD51; hRAD51
• UniProt Entry Name: RAD51_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Uniprot Description

Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028).