Western Blot (WB)
(Anti- Keap1 Picoband antibody, MBS178080, Western blottingAll lanes: Anti Keap1 (MBS178080) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugPredicted bind size: 70KDObserved bind size: 70KD )
anti-Human Keap1 Polyclonal Antibody | anti-KEAP1 antibody
Anti-Keap1 Antibody
Western Blot (WB)
(Anti- Keap1 Picoband antibody, MBS178080, Western blottingAll lanes: Anti Keap1 (MBS178080) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugPredicted bind size: 70KDObserved bind size: 70KD )
Western Blot (WB)
(Anti- Keap1 Picoband antibody, MBS178080, Western blottingAll lanes: Anti Keap1 (MBS178080) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugPredicted bind size: 70KDObserved bind size: 70KD )
Background: KEAP1 (KELCH-LIKE ECH-ASSOCIATED PROTEIN 1), is a protein that in humans is encoded by the Keap1 gene. The KIAA0132 gene is mapped on 19p13.2. Keap1 contains a central BTB/POZ domain and a C-terminal double glycine repeat (DGR), or Kelch, module. Keap1 has been shown to interact with Nrf2, a master regulator of the antioxidant response, which is important for the amelioration of oxidative stress. In the presence of the electrophilic agent diethylmalate, Nrf2 activity is released from Keap1 and Nrf2 translocate to the nucleus. Under quiescent conditions, Nrf2 is anchored in the cytoplasm through binding to Keap1, which, in turn, facilitates the ubiquitination and subsequent proteolysis of Nrf2. Because Nrf2 activation leads to a coordinated antioxidant and anti-inflammatory response, and Keap1 represses Nrf2 activation, Keap1 has become a very attractive drug target.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
KEAP1: a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Has tumor suppressor activity. Retains NRF2, BPTF and PGAM5 in the cytosol. Targets NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and of antioxidant response element-mediated detoxifying enzyme gene expression. Somatic mutations of KEAP1 or NRF2 commonly occur in solid cancers, irrespective of histological type, resulting in the constitutive transcription of cytoprotective genes. Genetic disruption of the KEAP1/CUL3 E3 ubiquitin ligase complex leads to the activation of the NF-kappaB pathway in lung cancer. Alternative activation of the cytoprotective NRF2 pathway exist in the absence of mutations in KEAP1 and HRF2: WTX, PALB2, SQSTM1, and DPP3 can competitively bind KEAP1. These proteins and others that bind KEAP1 contain an ¿ETGE¿ amino acid motif, which matches the KEAP1 interaction motif found in NRF2. Binding of these ¿ETGE¿-containing proteins can displace NRF2, freeing it to translocate to the nucleus and implement its cytoprotective transcriptional pathway. The DPP3 gene is frequently overexpressed in squamous cell lung carcinomas with high wt NRF2 activity. Its expression level significantly correlates with the response rate and progression-free survival of platinum-based first-line chemotherapy. May be a useful biomarker for predicting the chemosensitivity of patients with advanced-stage NSCLC. Broadly expressed, with highest levels in skeletal muscle. Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.
Protein type: Ubiquitin conjugating system; Endoplasmic reticulum; Transcription regulation; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: actin filament; cell-cell adherens junction; cytoplasm; cytosol; endoplasmic reticulum; focal adhesion; microtubule organizing center; midbody; nucleoplasm
Molecular Function: protein binding; transcription factor binding; ubiquitin-protein ligase activity
Biological Process: alkanesulfonate metabolic process; cytoplasmic sequestering of transcription factor; flavonoid metabolic process; in utero embryonic development; malate metabolic process; negative regulation of transcription factor activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-independent protein catabolic process; protein oligomerization; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of epidermal cell differentiation; response to metal ion; selenium metabolic process; transcription, DNA-dependent
Research Articles on KEAP1
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Product Notes
The KEAP1 keap1 (Catalog #AAA178080) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Keap1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Keap1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the KEAP1 keap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keap1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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