TSLP active protein

Recombinant Human TSLP (C-10His)

Cat. Num. AAA177943

• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: TSLP; N/A; Recombinant Human TSLP (C-10His); Thymic stromal lymphopoietin; TSLP active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Tyr29-Gln159

• Species: Human
• Tag: C-10His
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human Thymic Stromal lymphopoietin is produced by our Mammalian expression system and the target gene encoding Tyr29-Gln159 is expressed with a 10His tag at the C-terminus.
• Activity: Loaded Human TSLP-His on HIS1K Biosensor, can bind Human TSLP R-Fc with an affinity constant of 37.97nM as determined in BLI assay
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

Bioactivity

SDS-PAGE

Related Product Information for TSLP active protein

Thymic stromal lymphopoietin (TSLP) is a novel member of the hemopoietic cytokine family that promotes the development of B cells and shares overlapping activity with IL-7. The human TSLP protein comprises a 28 amino acids (aa) signal sequence and 131 aa mature region. Human TSLP has two isoforms lfTSLP and sfTSLP produced by alternative splicing. lfTSLP is expressed in a number of tissues including heart, liver and prostate, and sfTSLP (63aa) is predominantly expressed in keratinocytes of oral mucosa, skin and in salivary glands. In aa sequence level, Human TSLP displays about 43% identity with mouse TSLP.TSLP is a cytokine that functions mainly on myeloid cells; it induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells.TSLP has proliferative effects on the myeloid cell line and may initiate asthma or atopic dermatitis responses by directly activating mast cells. TSLP signals cells via the interleukin-7 receptor-alpha chain (IL-7Ralpha),shared with IL-7, together with the TSLP receptor (TSLPR) subunit. Recent studies indicate that TSLP and its receptor are novel therapeutic targets for rheumatoid arthritis,for increased intraarticular TSLP concentrations in patients has caused chemotaxis and activation of arthritogenic T cells.

Product Categories/Family for TSLP active protein

immunology

NCBI and Uniprot Product Information

• NCBI GI #: 190886449
• NCBI GeneID: 85480
• NCBI Accession #: NP_612561.2
• NCBI GenBank Nucleotide #: NM_138551.4
• UniProt Accession #: Q969D9; Q8IW99
• Molecular Weight: 18,141 Da
• NCBI Official Full Name: thymic stromal lymphopoietin isoform 2
• NCBI Official Synonym Full Names: thymic stromal lymphopoietin
• NCBI Official Symbol: TSLP
• NCBI Protein Information: thymic stromal lymphopoietin
• UniProt Protein Name: Thymic stromal lymphopoietin
• UniProt Gene Name: TSLP
• UniProt Entry Name: TSLP_HUMAN