Western Blot (WB)
(Anti- ANP Picoband antibody, MBS177925, Western blottingAll lanes: Anti ANP (MBS177925) at 0.5ug/mlLane 1: Rat Cardiac Muscle Tissue Lysate at 50ugLane 2: Mouse Cardiac Muscle Tissue Lysate at 50ugPredicted bind size: 17KDObserved bind size: 17KD )
ANP Polyclonal Antibody | anti-ANP antibody
Anti-ANP Antibody
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse, Rat
Predicted Species: Human
Antigen Retrieval: N/A
WB: The detection limit for ANP is approximately 0.25 ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions to be determined by end users.
Western Blot (WB)
(Anti- ANP Picoband antibody, MBS177925, Western blottingAll lanes: Anti ANP (MBS177925) at 0.5ug/mlLane 1: Rat Cardiac Muscle Tissue Lysate at 50ugLane 2: Mouse Cardiac Muscle Tissue Lysate at 50ugPredicted bind size: 17KDObserved bind size: 17KD )
Western Blot (WB)
(Anti- ANP Picoband antibody, MBS177925, Western blottingAll lanes: Anti ANP (MBS177925) at 0.5ug/mlLane 1: Rat Cardiac Muscle Tissue Lysate at 50ugLane 2: Mouse Cardiac Muscle Tissue Lysate at 50ugPredicted bind size: 17KDObserved bind size: 17KD )
Background: Atrial natriuretic peptide (ANP), also known as NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue). ANP is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney that is, aldosterone stimulates sodium retention and it generates sodium loss.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015]
Uniprot Description
NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family.
Protein type: Secreted, signal peptide; Hormone; Secreted
Chromosomal Location of Human Ortholog: 1p36.21
Cellular Component: extracellular region; extracellular space; mast cell granule; nucleus; perinuclear region of cytoplasm
Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; protein binding; receptor binding
Biological Process: cardiac muscle hypertrophy; cellular protein metabolic process; cGMP biosynthetic process; female pregnancy; negative regulation of cell growth; negative regulation of systemic arterial blood pressure; neuropeptide signaling pathway; positive regulation of heart rate; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; regulation of blood vessel size; response to hypoxia; response to insulin stimulus; transcription initiation from RNA polymerase II promoter
Disease: Atrial Fibrillation, Familial, 6; Atrial Standstill 2
Research Articles on ANP
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Product Notes
The ANP nppa (Catalog #AAA177925) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-ANP Antibody reacts with Mouse, Rat; Predicted to work with: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ANP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot -- Concentration: 0.1-0.5ug/ml Tested Species: Mouse, Rat Predicted Species: Human Antigen Retrieval: N/A WB: The detection limit for ANP is approximately 0.25 ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions to be determined by end users. Researchers should empirically determine the suitability of the ANP nppa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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