AIM2 Polyclonal Antibody | anti-AIM2 antibody

Anti-AIM2 Antibody

Cat. Num. AAA177917

• Gene Names: AIM2; PYHIN4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: AIM2; Polyclonal Antibody; Anti-AIM2 Antibody; Interferon-inducible protein AIM2; Absent in melanoma 2; AIM 2; Aim2; AIM2_HUMAN; OTTHUMP00000035296; PYHIN4; absent in melanoma 2; anti-AIM2 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 343

• Applicable Applications for anti-AIM2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human AIM2 recombinant protein (Position: L14-H215). Human AIM2 shares 53.8% amino acid (aa) sequence identity with mouse AIM2.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- AIM2 Picoband antibody, MBS177917, Western blottingAll lanes: Anti AIM2 (MBS177917) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugPredicted bind size: 39KDObserved bind size: 45KD )

Immunohistochemistry (IHC)

(Anti- AIM2 Picoband antibody, MBS177917, IHC(P)IHC(P): Mouse Kidney Tissue )

Immunohistochemistry (IHC)

(Anti- AIM2 Picoband antibody, MBS177917, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti- AIM2 Picoband antibody, MBS177917, IHC(P)IHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-AIM2 antibody

Description: Rabbit IgG polyclonal antibody for Interferon-inducible protein AIM2(AIM2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Interferon-inducible protein AIM2, also known as absent in melanoma 2 or simply AIM2, is a protein that in humans is encoded by the AIM2 gene. It is mapped to 1q22. AIM2 is a member of the Ifi202/IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNA (either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another protein ASC (or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains a CARD domain (caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation of caspase-1, an enzyme that processes proinflammatory cytokines (IL-1b and IL-18).

References

1. DeYoung KL, Ray ME, Su YA, Anzick SL, Johnstone RW, Trapani JA, Meltzer PS, Trent JM (Aug 1997). "Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of human melanoma". Oncogene 15 (4): 453-7. 2. "Entrez Gene: AIM2 absent in melanoma 2". 3. Woerner SM, Kloor M, Schwitalle Y, Youmans H, von Knebel Doeberitz M, Gebert J, Dihlmann S (2007). "The putative tumor suppressor AIM2 is frequently affected by different genetic alterations in microsatellite unstable colon cancers". Genes Chromosomes Cancer 46 (12): 1080-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4757734
• NCBI GeneID: 9447
• NCBI Accession #: NP_004824.1
• NCBI GenBank Nucleotide #: NM_004833.1
• UniProt Accession #: O14862; Q5T3V9; Q96FG9; A8K7M7
• Molecular Weight: 38,954 Da
• NCBI Official Full Name: interferon-inducible protein AIM2
• NCBI Official Synonym Full Names: absent in melanoma 2
• NCBI Official Symbol: AIM2
• NCBI Official Synonym Symbols: PYHIN4
• NCBI Protein Information: interferon-inducible protein AIM2
• UniProt Protein Name: Interferon-inducible protein AIM2
• Protein Family: Protein
• UniProt Gene Name: AIM2
• UniProt Entry Name: AIM2_HUMAN

NCBI Description

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq, Jul 2008]

Uniprot Description

AIM2: a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. Induced by interferon gamma. Defects in AIM2 may be a cause of microsatellite unstable colon cancers. AIM2 and various NOD-like receptors form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of pro-IL-1beta.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: double-stranded DNA binding; identical protein binding; protein binding

Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; apoptosis; immune response; inflammatory response; inhibition of NF-kappaB transcription factor; innate immune response; interleukin-1 beta secretion; positive regulation of defense response to virus by host; positive regulation of interleukin-1 beta production; positive regulation of interleukin-1 beta secretion; positive regulation of protein oligomerization; tumor necrosis factor-mediated signaling pathway