IKB beta Polyclonal Antibody | anti-IKB beta antibody

Anti-IKB beta Antibody

Cat. Num. AAA177911

• Gene Names: NFKBIB; IKBB; TRIP9
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: IKB beta; Polyclonal Antibody; Anti-IKB beta Antibody; NF-kappa-B inhibitor beta; I kappa B beta; I-kappa-B-beta; IkappaBbeta; IkB-B; IkB-beta; IKBB; IKBB_HUMAN; IkBbeta; NF kappa BIB; NF-kappa-BIB; Nfkbib; Thyroid receptor interacting protein 9; Thyroid receptor-interacting protein 9; TR interacting protein 9; TR-interacting protein 9; TRIP-9; TRIP9; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor; beta; anti-IKB beta antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 270

• Applicable Applications for anti-IKB beta antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human IKB beta recombinant protein (Position: E56-E237). Human IKB beta shares 82% and 80% amino acid (aa) sequence identity with mouse and rat IKB beta, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- IKB beta Picoband antibody, MBS177911, Western blottingAll lanes: Anti IKB beta (MBS177911) at 0.5ug/mlLane 1: Mouse Kidney Tissue Lysate at 50ugLane 2: RH35 Whole Cell Lysate at 40ugLane 3: NRK Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 38KDObserved bind size: 38KD )

Immunohistochemistry (IHC)

(Anti- IKB beta Picoband antibody, MBS177911,IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Immunohistochemistry (IHC)

(Anti- IKB beta Picoband antibody, MBS177911,IHC(P)IHC(P): Mouse Intestine Tissue )

Immunohistochemistry (IHC)

(Anti- IKB beta Picoband antibody, MBS177911,IHC(P)IHC(P): Rat Intestine Tissue )

Related Product Information for anti-IKB beta antibody

Description: Rabbit IgG polyclonal antibody for NF-kappa-B inhibitor beta(NFKBIB) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: NF-kappa-B inhibitor beta, also known as IKBB or TRIP9, is a protein that in humans is encoded by the NFKBIB gene. The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. This gene is mapped to 19q13.2. It has been found that in vivo, NFKBIB serves both to inhibit and to facilitate the inflammatory response. NFKBIB degradation releases NF-kappa-B dimers, which upregulate proinflammatory target genes such as TNF-alpha. Surprisingly, absence of NFKBIB results in a dramatic reduction of TNF-alpha in response to lipopolysaccharide, even though activation of NF-kappa-B is normal.

References

1. Okamoto T, Ono T, Hori M, Yang J, Tetsuka T, Kawabe T et al. (November 1998). "Assignment of the IkappaB-beta gene NFKBIB to human chromosome band 19q13.1 by in situ hybridization". Cytogenet Cell Genet 82 (1-2): 105-6. 2. Rao, P., Hayden, M. S., Long, M., Scott, M. L., West, A. P., Zhang, D., Oeckinghaus, A., Lynch, C., Hoffmann, A., Baltimore, D., Ghosh, S. I-kappa-B-beta acts to inhibit and activate gene expression during the inflammatory response. Nature 466: 1115-1119, 2010.

NCBI and Uniprot Product Information

• NCBI GI #: 340805857
• NCBI GeneID: 4793
• NCBI Accession #: NP_001230045.1
• NCBI GenBank Nucleotide #: NM_001243116.1
• UniProt Accession #: Q15653; Q96BJ7; A8K3F4
• Molecular Weight: 35,530 Da
• NCBI Official Full Name: NF-kappa-B inhibitor beta isoform 2
• NCBI Official Synonym Full Names: NFKB inhibitor beta
• NCBI Official Symbol: NFKBIB
• NCBI Official Synonym Symbols: IKBB; TRIP9
• NCBI Protein Information: NF-kappa-B inhibitor beta
• UniProt Protein Name: NF-kappa-B inhibitor beta
• UniProt Gene Name: NFKBIB
• UniProt Synonym Gene Names: IKBB; TRIP9; NF-kappa-BIB; IkB-B; IkB-beta; IkappaBbeta; TR-interacting protein 9; TRIP-9
• UniProt Entry Name: IKBB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

IkB-beta: a protein of the NF-kappa-B inhibitor family. Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA- dependent inactivation. Association with inhibitor kappa B- interacting Ras-like proteins 1 and 2 may also explain the slower degradation. Interacts with ligand-binding domain of thyroid hormone receptor-beta, inhibitor kappa B-interacting Ras-like protein 1, inhibitor kappa B-interacting protein Ras2, p65 (RELA) and c-Rel. Expressed in all tissues examined.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; signal transducer activity; transcription coactivator activity

Biological Process: activation of NF-kappaB transcription factor; cytoplasmic sequestering of NF-kappaB; signal transduction; transcription, DNA-dependent