anti-Mouse, Rat MAD1 Polyclonal Antibody | anti-MAD1 antibody

Anti-MAD1 Antibody

Cat. Num. AAA177892

• Gene Names: MAD1L1; MAD1; PIG9; TP53I9; TXBP181
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: MAD1; Polyclonal Antibody; Anti-MAD1 Antibody; Mitotic spindle assembly checkpoint protein MAD1; hMAD1; HsMAD1; MAD1 mitotic arrest deficient like 1 (yeast); MAD1-like protein 1; MAD1L1; MD1L1_HUMAN; Mitotic arrest deficient 1; Mitotic arrest deficient 1-like protein 1; Mitotic checkpoint MAD1 protein homolog; PIG9; Tax binding protein 181; Tax-binding protein 181; TP53I9; Tumor protein p53 inducible protein 9; TXBP181; MAD1 mitotic arrest deficient-like 1 (yeast); anti-MAD1 antibody

• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 718

• Applicable Applications for anti-MAD1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human MAD1 recombinant protein (Position: L362-A632). Human MAD1 shares 81% amino acid (aa) sequence identity with mouse MAD1.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- MAD1 Picoband antibody, MBS177892, Western blottingAll lanes: Anti MAD1 (MBS177892) at 0.5ug/mlWB: Recombinant Human MAD1 Protein 0.5ngPredicted bind size: 50KDObserved bind size: 50KD )

Western Blot (WB)

(Anti- MAD1 Picoband antibody, MBS177892, Western blottingAll lanes: Anti MAD1 (MBS177892) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: JURKAT Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: 293T Whole Cell Lysate at 40ugLane 5: SHG Whole Cell Lysate at 40ugLane 6: 22RV1 Whole Cell Lysate at 40ugLane 7: PANC Whole Cell Lysate at 40ugPredicted bind size: 83KDObserved bind size: 83KD )

Immunohistochemistry (IHC)

(Anti- MAD1 Picoband antibody, MBS177892, IHC(P)IHC(P): Human Placenta Tissue )

Related Product Information for anti-MAD1 antibody

Description: Rabbit IgG polyclonal antibody for Mitotic spindle assembly checkpoint protein MAD1(MAD1L1) detection. Tested with WB, IHC-P in Human;Mouse;Rat

Background: Mitotic spindle assembly checkpoint protein MAD1 is a protein that in humans is encoded by the MAD1L1 gene. It is mapped to 7p22.3. MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 can function as a homodimer. It localizes to the centrosome during metaphase and to the spindle midzone and the midbody during anaphase and telophase. MAD1L1 may also play a role in cell cycle control and tumor suppression.

References

1. "Entrez Gene: MAD1L1 MAD1 mitotic arrest deficient-like 1 (yeast)" 2. Jin, D.-Y., Kozak, C. A., Pangilinan, F., Spencer, F., Green, E. D., Jeang, K.-T. Mitotic checkpoint locus MAD1L1 maps to human chromosome 7p22 and mouse chromosome 5. Genomics 55: 363-364, 1999. 3. Jin, D.-Y., Spencer, F., Jeang, K.-T. Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1. Cell 93: 81-91, 1998. 4. Tsukasaki, K., Miller, C. W., Greenspun, E., Eshaghian, S., Kawabata, H., Fujimoto, T., Tomonaga, M., Sawyers, C., Said, J. W., Koeffler, H. P. Mutations in the mitotic check point gene, MAD1L1, in human cancers. Oncogene 20: 3301-3305, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 62243369
• NCBI GeneID: 8379
• NCBI Accession #: NP_001013858.1
• NCBI GenBank Nucleotide #: NM_001013836.1
• UniProt Accession #: Q9Y6D9; Q13312; Q75MI0; Q86UM4; Q9UNH0; B3KR41
• Molecular Weight: 72,286 Da
• NCBI Official Full Name: mitotic spindle assembly checkpoint protein MAD1 isoform a
• NCBI Official Synonym Full Names: MAD1 mitotic arrest deficient like 1
• NCBI Official Symbol: MAD1L1
• NCBI Official Synonym Symbols: MAD1; PIG9; TP53I9; TXBP181
• NCBI Protein Information: mitotic spindle assembly checkpoint protein MAD1
• UniProt Protein Name: Mitotic spindle assembly checkpoint protein MAD1
• Protein Family: Mitotic spindle checkpoint protein
• UniProt Gene Name: MAD1L1
• UniProt Synonym Gene Names: MAD1; TXBP181; MAD1-like protein 1; HsMAD1; hMAD1
• UniProt Entry Name: MD1L1_HUMAN

NCBI Description

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

MAD1L1: a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Expressed weakly at G0/G1 and highly at late S and G2/M phase. Induced by p53. Becomes hyperphosphorylated in late S through M phases or after mitotic spindle damage. Two alternatively spliced isoforms have been described. Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.

Protein type: Cytoskeletal; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: actin cytoskeleton; centrosome; cytoplasm; cytosol; kinetochore; nuclear pore; nucleus; spindle; spindle pole

Molecular Function: protein binding

Biological Process: cell division; mitosis; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; negative regulation of T cell proliferation; sister chromatid cohesion; thymus development

Disease: Prostate Cancer