Western Blot (WB)
(Anti- RALA Picoband antibody, MBS177874, Western blottingAll lanes: Anti RALA (MBS177874) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Thymus Tissue Lysate at 50ugLane 3: Rat Testis Tissue Lysate at 50ugLane 4: Mouse Thymus Tissue Lysate at 50ugLane 5: Mouse Liver Tissue Lysate at 50ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 24KDObserved bind size: 24KD )
RALA Polyclonal Antibody | anti-RALA antibody
Anti-RALA Antibody
Western Blot (WB)
(Anti- RALA Picoband antibody, MBS177874, Western blottingAll lanes: Anti RALA (MBS177874) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Thymus Tissue Lysate at 50ugLane 3: Rat Testis Tissue Lysate at 50ugLane 4: Mouse Thymus Tissue Lysate at 50ugLane 5: Mouse Liver Tissue Lysate at 50ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 24KDObserved bind size: 24KD )
Western Blot (WB)
(Anti- RALA Picoband antibody, MBS177874, Western blottingAll lanes: Anti RALA (MBS177874) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Thymus Tissue Lysate at 50ugLane 3: Rat Testis Tissue Lysate at 50ugLane 4: Mouse Thymus Tissue Lysate at 50ugLane 5: Mouse Liver Tissue Lysate at 50ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 24KDObserved bind size: 24KD )
Background: Ras-related protein Ral-A (RalA) is a protein that in humans is encoded by the RALA gene on chromosome 7. This protein is one of two isoforms of the Ral protein, the other being RalB, and part of the Ras GTPase family. As a Ras GTPase, RalA functions as a molecular switch that becomes active when bound to GTP and inactive when bound to GDP. It can be activated by RalGEFs and, in turn, activate effectors in signal transduction pathways leading to biological outcomes. Additionally, Ral proteins have been associated with the progression of several cancers, including bladder cancer and prostate cancer. While the above functions appear to be shared between the two Ral isoforms, their differential subcellular localizations result in their differing involvement in certain biological processes. In particular, RalA is more involved in anchorage-independent cell growth, vesicle trafficking, and cytoskeletal organization. Moreover, RalA specifically interacts with Exo84 and Sec5 to regulate transport of membrane proteins in polarized epithelial cells and GLUT4 to the plasma membrane, as well as mitochondrial fission for cell division.
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. [provided by RefSeq, Jul 2008]
Uniprot Description
RALA: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1. Activated in an LPA-dependent manner by LPAR1 and in an LPA-independent manner by LPAR2. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.
Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, Ras; G protein
Chromosomal Location of Human Ortholog: 7p15-p13
Cellular Component: cell surface; cleavage furrow; cytoplasmic vesicle membrane; endocytic vesicle; focal adhesion; midbody; myelin sheath; plasma membrane
Molecular Function: ATPase binding; Edg-2 lysophosphatidic acid receptor binding; GDP binding; GTP binding; GTPase activity; myosin binding; protein binding; ubiquitin protein ligase binding
Biological Process: actin cytoskeleton reorganization; chemotaxis; cytokinesis; exocytosis; intracellular protein transport; lipid raft localization; metabolic process; neural tube closure; nucleocytoplasmic transport; positive regulation of filopodium formation; Ras protein signal transduction; regulation of exocytosis; signal transduction; viral reproduction
Research Articles on RALA
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Product Notes
The RALA rala (Catalog #AAA177874) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-RALA Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RALA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the RALA rala for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RALA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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