MNAT1 Polyclonal Antibody | anti-MNAT1 antibody

Anti-MNAT1 Antibody

Cat. Num. AAA177855

• Gene Names: MNAT1; MAT1; TFB3; CAP35; RNF66
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: MNAT1; Polyclonal Antibody; Anti-MNAT1 Antibody; CDK-activating kinase assembly factor MAT1; MNAT 1; CAP35; CDK activating kinase assembly factor MAT1; CDK7/cyclin H assembly factor; CDK7/cyclin-H assembly factor; Cyclin G1 interacting protein; Cyclin-G1-interacting protein; MAT1; MAT1_HUMAN; Menage a trois 1 (CAK assembly factor); Menage a trois; Menage a trois homolog 1; cyclin H assembly factor (Xenopus laevis); MNAT CDK activating kinase assembly factor 1; Mnat1; p35; p36; RING finger protein 66; RING finger protein MAT1; RNF66; TFB3; MNAT CDK-activating kinase assembly factor 1; anti-MNAT1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 267

• Applicable Applications for anti-MNAT1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human MNAT1 recombinant protein (Position: L92-A278). Human MNAT1 shares 93% amino acid (aa) sequence identity with mouse MNAT1.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- MNAT1 Picoband antibody, MBS177855, Western blottingAll lanes: Anti MNAT1 (MBS177855) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: MCF-7 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 81KDObserved bind size: 81KD )

Immunohistochemistry (IHC)

(Anti- MNAT1 Picoband antibody, MBS177855, IHC(P)IHC(P): Mouse Testis Tissue )

Immunohistochemistry (IHC)

(Anti- MNAT1 Picoband antibody, MBS177855, IHC(P)IHC(P): Rat Testis Tissue )

Immunohistochemistry (IHC)

(Anti- MNAT1 Picoband antibody, MBS177855, IHC(P)IHC(P): Human Testis Tissue )

Related Product Information for anti-MNAT1 antibody

Description: Rabbit IgG polyclonal antibody for CDK-activating kinase assembly factor MAT1(MNAT1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: CDK-activating kinase assembly factor MAT1 is an enzyme that in humans is encoded by the MNAT1 gene. The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene.

References

1. "Entrez Gene: MNAT1 menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)". 2. Eki T, Okumura K, Abe M, Kagotani K, Taguchi H, Murakami Y, Pan ZQ, Hanaoka F (April 1998). "Mapping of the human genes encoding cyclin H (CCNH) and the CDK-activating kinase (CAK) assembly factor MAT1 (MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively". Genomics 47 (1): 115-20. 3. Talukder AH, Mishra SK, Mandal M, Balasenthil S, Mehta S, Sahin AA, Barnes CJ, Kumar R (March 2003). "MTA1 interacts with MAT1, a cyclin-dependent kinase-activating kinase complex ring finger factor, and regulates estrogen receptor transactivation functions". J. Biol. Chem. 278 (13): 11676-85.

NCBI and Uniprot Product Information

• NCBI GI #: 295789085
• NCBI GeneID: 4331
• NCBI Accession #: NP_001171434.1
• NCBI GenBank Nucleotide #: NM_001177963.1
• UniProt Accession #: P51948; Q15817; Q6ICQ7; G3V1U8
• Molecular Weight: 31,148 Da
• NCBI Official Full Name: CDK-activating kinase assembly factor MAT1 isoform 2
• NCBI Official Synonym Full Names: MNAT1, CDK activating kinase assembly factor
• NCBI Official Symbol: MNAT1
• NCBI Official Synonym Symbols: MAT1; TFB3; CAP35; RNF66
• NCBI Protein Information: CDK-activating kinase assembly factor MAT1
• UniProt Protein Name: CDK-activating kinase assembly factor MAT1
• Protein Family: CDK-activating kinase assembly factor
• UniProt Gene Name: MNAT1
• UniProt Synonym Gene Names: CAP35; MAT1; RNF66
• UniProt Entry Name: MAT1_HUMAN

NCBI Description

The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.