anti-Human, Rat Caspase-7 Polyclonal Antibody | anti-CASP7 antibody

Anti-Caspase-7 Antibody

Cat. Num. AAA177767

• Gene Names: CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Caspase-7; Polyclonal Antibody; Anti-Caspase-7 Antibody; Apoptosis related cysteine peptidase; Apoptosis Related Cysteine Protease; Apoptotic protease Mch-3; Apoptotic Protease MCH3; CASP 7; CASP-7; CASP7; CASP7_HUMAN; Caspase 7; Caspase 7 apoptosis related cysteine peptidase; Caspase-7 subunit p11; Caspase7; CMH 1; CMH-1; CMH1; ICE LAP3; ICE like Apoptotic Protease 3; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; MCH3; caspase 7; apoptosis-related cysteine peptidase; anti-CASP7 antibody

• Reactivity: Human, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 303

• Applicable Applications for anti-CASP7 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human Caspase-7 recombinant protein (Position: A117-D198). Human Caspase-7 shares 92.7% amino acid (aa) sequence identity with both mouse and rat Caspase-7.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Caspase-7 Picoband antibody, MBS177767, Western blottingAll lanes: Anti Caspase-7(MBS177767) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: Rat Spleen Tissue Lysate at 50ugLane 3: Rat Lung Tissue Lysate at 50ugPredicted bind size: 34KDObserved bind size: 34KD )

Immunohistochemistry (IHC)

(Anti- Caspase-7 Picoband antibody, MBS177767,IHC(P)IHC(P): Human Mammary Cancer Tissue )

Related Product Information for anti-CASP7 antibody

Description: Rabbit IgG polyclonal antibody for Caspase-7(CASP7) detection. Tested with WB, IHC-P in Human;Rat.

Background: CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway.

References

1. Burguillos, M. A., Deierborg, T., Kavanagh, E., Persson, A., Hajji, N., Garcia-Quintanilla, A., Cano, J., Brundin, P., Englund, E., Venero, J. L., Joseph, B. Caspase signalling controls microglia activation and neurotoxicity. Nature 472: 319-324, 2011. 2. Soung, Y. H., Lee, J. W., Kim, H. S., Park, W. S., Kim, S. Y., Lee, J. H., Park, J. Y., Cho, Y. G., Kim, C. J., Park, Y. G., Nam, S. W., Jeong, S. W., Kim, S. H., Lee, J. Y., Yoo, N. J., Lee, S. H. Inactivating mutations of CASPASE-7 gene in human cancers. Oncogene 22: 8048-8052, 2003. 3. Tiso, N., Pallavicini, A., Muraro, T., Zimbello, R., Apolloni, E., Valle, G., Lanfranchi, G., Danieli, G. A. Chromosomal localization of the human genes, CPP32, Mch2, Mch3, and Ich-1, involved in cellular apoptosis. Biochem. Biophys. Res. Commun. 225: 983-989, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 4502581
• NCBI GeneID: 840
• NCBI Accession #: NP_001218.1
• NCBI GenBank Nucleotide #: NM_001227.4
• UniProt Accession #: P55210; Q13364; Q53YD5; Q5SVL0; Q5SVL3; Q96BA0; B4DQU7; B5BU45; D3DRB8
• Molecular Weight: 31,614 Da
• NCBI Official Full Name: caspase-7 isoform alpha
• NCBI Official Synonym Full Names: caspase 7
• NCBI Official Symbol: CASP7
• NCBI Official Synonym Symbols: MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
• NCBI Protein Information: caspase-7
• UniProt Protein Name: Caspase-7
• Protein Family: Caspase
• UniProt Gene Name: CASP7
• UniProt Synonym Gene Names: MCH3; CASP-7; ICE-LAP3
• UniProt Entry Name: CASP7_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.