Typical Testing Data/Standard Curve (for reference only)
Cation-independent mannose-6-phosphate receptor ELISA Kit | IGF2R elisa kit
Human IGF2R PicoKine ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TGF-beta-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TGF-beta-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TGF-beta-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele. [provided by RefSeq, Apr 2013]
Uniprot Description
IGF2R: a multifunctional type I transmembrane protein receptor that binds insulin-like growth factor 2 at the cell surface and mannose-6-phosphate-tagged proteins in the trans-Golgi network. Also known as the cation-independent mannose-6 phosphate (M6P) receptor. Clears IGF2 from the cell surface to attenuate signaling, and transports M6P-tagged lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. M6P-tagged lysosomal enzymes bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Belongs to the MRL1/IGF2R family.
Protein type: Receptor, misc.; Membrane protein, integral; Vesicle
Chromosomal Location of Human Ortholog: 6q26
Cellular Component: extracellular space; clathrin coat; cell surface; focal adhesion; lysosomal membrane; integral to plasma membrane; trans-Golgi network; trans-Golgi network transport vesicle; membrane; perinuclear region of cytoplasm; endocytic vesicle; nuclear envelope lumen; late endosome; endosome
Molecular Function: G-protein coupled receptor activity; identical protein binding; mannose binding; protein binding; enzyme binding; retinoic acid binding; transporter activity; phosphoprotein binding; G-protein alpha-subunit binding; receptor activity; glycoprotein binding; insulin-like growth factor II binding; insulin-like growth factor receptor activity
Biological Process: receptor-mediated endocytosis; G-protein coupled receptor protein signaling pathway; organ regeneration; response to retinoic acid; insulin-like growth factor receptor signaling pathway; positive regulation of apoptosis; spermatogenesis; liver development; signal transduction; post-embryonic development
Disease: Hepatocellular Carcinoma
Research Articles on IGF2R
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Product Notes
The Human. There is no detectable cross-reactivity with other relevant proteins. IGF2R igf2r (Catalog #AAA177544) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177544 ELISA Kit recognizes Human. There is no detectable cross-reactivity with other relevant proteins. IGF2R. It is sometimes possible for the material contained within the vial of "Cation-independent mannose-6-phosphate receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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