Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFR2 PicoKine ELISA Kit standard curve )
Mouse TNFR2 ELISA Kit | TNFRSF1B elisa kit
Mouse TNFR2 PicoKine ELISA Kit
Gene Names
Tnfrsf1b; p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2
Reactivity
Mouse
Synonyms
TNFR2; Mouse TNFR2 PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily; member 1B; CD120b; Etanercept; INN=Etanercept; p75; p80 TNF alpha receptor; TNF R2; TNFBR; TNFR-II; TNFRII; Tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor type II; TNFRSF1B elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse TNFR2
Sequence Length
474
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFR2 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFR2 PicoKine ELISA Kit standard curve )
Related Product Information for TNFRSF1B elisa kit
Principle of the assay: mouse TNFR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFR2 has been precoated onto 96-well plates. Standards(NSO, V23-G258) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFR2 amount of sample captured in plate.
Background: Tumor necrosis factor receptor 2 (TNFR2) is one of receptors of TNF. TNF has proinflammatory and immunosuppressive properties that may segregate at the level of the 2 TNF receptors (TNFRs), TNFR1 and TNFR2. The genes for TNFR1, a 55-kDa protein, and TNFR2, a 70-kDa protein, have been mapped to human chromosomes 12 (12pter-cen) and 1 (1pter-p32), respectively. TNFR2 was induced on glomerular endothelial cells of nephritic kidneys, and TNFR2 expression on intrinsic cells, but not leukocytes, was essential for glomerulonephritis and glomerular complement deposition. TNFR1 promotes systemic immune responses and renal T cell death, while intrinsic cell TNFR2 plays a critical role in complement-dependent tissue injury. Therefore, therapeutic blockade specifically of TNFR2 may be a promising strategy in the treatment of immune-mediated glomerulonephritis.
Background: Tumor necrosis factor receptor 2 (TNFR2) is one of receptors of TNF. TNF has proinflammatory and immunosuppressive properties that may segregate at the level of the 2 TNF receptors (TNFRs), TNFR1 and TNFR2. The genes for TNFR1, a 55-kDa protein, and TNFR2, a 70-kDa protein, have been mapped to human chromosomes 12 (12pter-cen) and 1 (1pter-p32), respectively. TNFR2 was induced on glomerular endothelial cells of nephritic kidneys, and TNFR2 expression on intrinsic cells, but not leukocytes, was essential for glomerulonephritis and glomerular complement deposition. TNFR1 promotes systemic immune responses and renal T cell death, while intrinsic cell TNFR2 plays a critical role in complement-dependent tissue injury. Therefore, therapeutic blockade specifically of TNFR2 may be a promising strategy in the treatment of immune-mediated glomerulonephritis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50,320 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 1B
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 1b
NCBI Official Symbol
Tnfrsf1b
NCBI Official Synonym Symbols
p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2
NCBI Protein Information
tumor necrosis factor receptor superfamily member 1B; TNF-RII; TNFR-II; p75 TNFR; p80 TNF-alpha receptor; TNF receptor beta chain; tumor necrosis factor receptor 2; tumor necrosis factor receptor type II
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 1B
Protein Family
UniProt Gene Name
Tnfrsf1b
UniProt Synonym Gene Names
Tnfr-2; Tnfr2; TNF-R2; TNF-RII; TNFR-II
UniProt Entry Name
TNR1B_MOUSE
Uniprot Description
TNF-R2: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Binds to TRAF2. Interacts with BMX. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Cellular Component: cell soma; membrane; axon; perinuclear region of cytoplasm; integral to membrane; intracellular; nucleus; lipid raft
Molecular Function: protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding
Biological Process: cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction resulting in induction of apoptosis; tumor necrosis factor-mediated signaling pathway; negative regulation of inflammatory response; programmed cell death; immune response; inflammatory response; RNA destabilization; positive regulation of membrane protein ectodomain proteolysis
Research Articles on TNFRSF1B
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Product Notes
The Mouse TNFRSF1B tnfrsf1b (Catalog #AAA177242) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177242 ELISA Kit recognizes Mouse TNFRSF1B. It is sometimes possible for the material contained within the vial of "TNFR2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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