Rabbit Huntingtin Polyclonal Antibody | anti-HTT antibody

Anti-Huntingtin antibody

Cat. Num. AAA176876

• Gene Names: HTT; HD; IT15
• Reactivity: Human; Predicted: Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified
• Synonyms: Huntingtin; Polyclonal Antibody; Anti-Huntingtin antibody; huntingtin; AI256365 antibody; C430023I11Rik antibody; HD antibody; HD protein antibody; HDH antibody; HTT antibody; Huntingtin antibody; HUNTINGTON CHOREA antibody; Huntington disease protein antibody; IT 15 antibody; IT15 antibody; OTTMUSP00000026909 antibody; ZHD antibody; anti-HTT antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified
• Form/Format: Lyophilized; Contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg Thimerosal, 0.05mg NaN₃

• Applicable Applications for anti-HTT antibody: Western Blot (WB)
• Application Notes: Western Blot - ; Concentration: 0.1-0.5ug/ml; Tested Species: Human; Predicted Species: Mouse, Rat; Antigen Retrieval: N/A; ; Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; ; Other applications have not been tested. Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HTT (3106-3121aa ELDRRAFQSVLEVVAA), different from the related mouse and rat sequences by two amino acids.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.

Western Blot (WB)

(Anti-Huntingtin antibody, MBS176876, All Western blottingAll lanes: Anti-HTT(MBS176876) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: U87 Whole Cell Lysate at 40ugPredicted bind size: 348KDObserved bind size: 348KD)

Related Product Information for anti-HTT antibody

The huntingtin gene, also called HTT or HD(Huntington disease) gene, is the IT15("interesting transcript 15") gene which codes for a protein called the huntingtin protein. It is mapped to 4p16.3. The protein has no sequence homology with other proteins and is highly expressed in neurons and tests in humans and rodents. HTT upregulates the expression of Brain Derived Neurotrophic Factor(BDNF) at the transcription level, and this gene is required for normal development. The HTT protein is involved in vesicle trafficking as it interacts with HIP1, a clathrin-binding protein, to mediate endocytosis, the absorption of materials into a cell. HTT was also visualized as punctate staining likely to represent nerve endings. What's more, wildtype HTT may function in the nucleus in the assembly of nuclear matrix-bound protein complexes involved with transcriptional repression and RNA processing.

NCBI and Uniprot Product Information

• NCBI GI #: 296434520
• NCBI GeneID: 3064
• UniProt Accession #: P42858; Q9UQB7
• NCBI Official Full Name: Huntingtin
• NCBI Official Synonym Full Names: huntingtin
• NCBI Official Symbol: HTT
• NCBI Official Synonym Symbols: HD; IT15
• NCBI Protein Information: huntingtin; huntington disease protein
• UniProt Protein Name: Huntingtin
• Protein Family: HD protein
• UniProt Gene Name: HTT
• UniProt Synonym Gene Names: HD; IT15; HD protein
• UniProt Entry Name: HD_HUMAN

NCBI Description

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq, Jul 2008]

Uniprot Description

Huntingtin: may play a role in microtubule-mediated transport or vesicle function. Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. Defects are the cause of Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the Huntingtin gene, which translates as a polyglutamine repeat in the protein product. The Huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The Huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated isoforms displaying different relative abundance in various fetal and adult tissues.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; Golgi apparatus; protein complex; cytoplasmic vesicle membrane; mitochondrion; axon; endoplasmic reticulum; late endosome; dendrite; cytoplasm; autophagic vacuole; inclusion body; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; p53 binding; dynein intermediate chain binding; beta-tubulin binding; diazepam binding; transcription factor binding

Biological Process: ER to Golgi vesicle-mediated transport; citrulline metabolic process; paraxial mesoderm formation; regulation of protein phosphatase type 2A activity; regulation of synaptic plasticity; locomotory behavior; determination of adult life span; endosome transport; anterior/posterior pattern formation; L-glutamate import; regulation of mitochondrial membrane potential; establishment of mitotic spindle orientation; protein import into nucleus; organ development; quinolinate biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; vesicle transport along microtubule; visual learning; negative regulation of neuron apoptosis; Golgi organization and biogenesis; grooming behavior; endoplasmic reticulum organization and biogenesis; positive regulation of inositol-1,4,5-triphosphate receptor activity; striatum development; axon cargo transport; cell aging; olfactory lobe development; social behavior; lactate biosynthetic process from pyruvate; neuron apoptosis; iron ion homeostasis; insulin secretion; dopamine receptor signaling pathway; hormone metabolic process; neuron development; spermatogenesis; regulation of mitochondrial membrane permeability; response to calcium ion; neural plate formation; urea cycle

Disease: Huntington Disease