Rabbit Adiponectin Polyclonal Antibody | anti-ADIPOQ antibody

Anti-Adiponectin antibody

Cat. Num. AAA176872

• Gene Names: ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Adiponectin; Polyclonal Antibody; Anti-Adiponectin antibody; adiponectin; C1Q and collagen domain containing; 30 kDa adipocyte complement related protein antibody; 30 kDa adipocyte complement-related protein antibody; ACDC antibody; ACRP 30 antibody; ACRP30 antibody; ADIPO_HUMAN antibody; Adipocyte antibody; Adipocyte C1q and collagen domain containing protein antibody; Adipocyte complement related 30 kDa protein antibody; Adipocyte complement related protein of 30 kDa antibody; Adipocyte complement-related 30 kDa protein antibody; Adiponectin antibody; AdipoQ antibody; Adipose most abundant gene transcript 1 antibody; Adipose most abundant gene transcript 1 protein antibody; Adipose specific collagen like factor antibody; ADIPQTL1 antibody; ADPN antibody; APM 1 antibody; apM-1 antibody; ApM1 antibody; C1q and collagen domain-containing protein antibody; GBP 28 antibody; GBP28 antibody; Gelatin binding protein antibody; Gelatin binding protein 28 antibody; Gelatin-binding protein antibody; anti-ADIPOQ antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 244

• Applicable Applications for anti-ADIPOQ antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, ELISA (EIA)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ADIPOQ (224-244aa LYADNDNDSTFTGFLLYHDTN), different from the related mouse and rat sequences by one amino acid.
• Contents: Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Adiponectin antibody, MBS176872, Western blottingWB: Rat Heart Tissue Lysate )

Western Blot (WB)

(Anti-Adiponectin antibody, MBS176872, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human ADIPOQ, 44.4 KD(162aa tag+ M1-N244)Lane 1: Recombinant Human ADIPOQ Protein 10ngLane 2: Recombinant Human ADIPOQ Protein 5ngLane 3: Recombinant Human ADIPOQ Protein 2.5ng )

Immunohistochemistry (IHC)

(Anti-Adiponectin antibody, MBS176872, IHC(P)IHC(P): Rat Kidney Tissue )

Immunohistochemistry (IHC)

(Anti-Adiponectin antibody, MBS176872, IHC(P)IHC(P): Mouse Kidney Tissue )

Immunohistochemistry (IHC)

(Anti-Adiponectin antibody, MBS176872, IHC(F)IHC(F): Rat Liver Tissue )

Immunohistochemistry (IHC)

(Anti-Adiponectin antibody, MBS176872, IHC(P)IHC(P): Human Placenta Tissue )

Related Product Information for anti-ADIPOQ antibody

Description: Rabbit IgG polyclonal antibody for Adiponectin(ADIPOQ) detection. Tested with WB, IHC-P, IHC-F, ELISA in Human, Mouse, Rat.
Background: ADIPOQ(Adipocyte-, C1q-, and Collagen Domain-Containing), also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al.(2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al.(1999) showed that APM1 is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, they found links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Adiponectin is exclusively secreted from adipose tissue(and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.

NCBI and Uniprot Product Information

• NCBI GI #: 2493789
• NCBI GeneID: 9370
• UniProt Accession #: Q15848; Q58EX9
• Molecular Weight: 26,414 Da
• NCBI Official Full Name: Adiponectin
• NCBI Official Synonym Full Names: adiponectin, C1Q and collagen domain containing
• NCBI Official Symbol: ADIPOQ
• NCBI Official Synonym Symbols: ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
• NCBI Protein Information: adiponectin; gelatin-binding protein 28; adipose specific collagen-like factor; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipose most abundant gene transcript 1 protein
• UniProt Protein Name: Adiponectin
• Protein Family: Adiponectin
• UniProt Gene Name: ADIPOQ
• UniProt Synonym Gene Names: ACDC; ACRP30; APM1; GBP28; ACRP30; apM-1
• UniProt Entry Name: ADIPO_HUMAN

NCBI Description

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]

Uniprot Description

adiponectin: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Synthesized exclusively by adipocytes and secreted into plasma.

Protein type: Secreted, signal peptide; Endoplasmic reticulum; Secreted; Hormone

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; collagen; cell surface; endoplasmic reticulum; extracellular region

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; hormone activity; cytokine activity; sialic acid binding; receptor binding

Biological Process: circadian rhythm; negative regulation of MAP kinase activity; negative regulation of phagocytosis; negative regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; response to glucocorticoid stimulus; membrane hyperpolarization; positive regulation of cellular protein metabolic process; negative regulation of smooth muscle cell migration; glucose homeostasis; negative regulation of granulocyte differentiation; positive regulation of interleukin-8 production; positive regulation of glucose import; negative regulation of gluconeogenesis; response to glucose stimulus; adiponectin-mediated signaling pathway; negative regulation of protein amino acid autophosphorylation; negative regulation of blood pressure; negative regulation of cell migration; protein homooligomerization; response to nutrient; generation of precursor metabolites and energy; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of signal transduction; negative regulation of heterotypic cell-cell adhesion; glucose metabolic process; negative regulation of tumor necrosis factor production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of fat cell differentiation; negative regulation of synaptic transmission; response to sucrose stimulus; membrane depolarization; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of fatty acid metabolic process; fatty acid beta-oxidation; response to ethanol; cellular response to insulin stimulus; negative regulation of macrophage differentiation; negative regulation of low-density lipoprotein receptor biosynthetic process; negative regulation of inflammatory response; brown fat cell differentiation; response to hypoxia; fatty acid oxidation; positive regulation of protein amino acid phosphorylation; response to activity; negative regulation of transcription, DNA-dependent; positive regulation of myeloid cell apoptosis; positive regulation of blood pressure

Disease: Adiponectin, Serum Level Of, Quantitative Trait Locus 1