Human CCL19/MIP-3 beta ELISA Kit | CCL19 elisa kit

Human CCL19/MIP-3 beta PicoKine ELISA Kit

Cat. Num. AAA176518

• Gene Names: CCL19; ELC; CKb11; MIP3B; MIP-3b; SCYA19
• Reactivity: Human
• Synonyms: CCL19/MIP-3 beta; Human CCL19/MIP-3 beta PicoKine ELISA Kit; C-C motif chemokine 19; chemokine (C-C motif) ligand 19; Beta chemokine exodus 3; Beta-chemokine exodus-3; C C chemokine ligand 19; CC chemokine ligand 19; CCL19; CCL19_HUMAN; Chemokine (C C motif) ligand 19; Chemokine (CC motif) ligand 19; Chemokine CC Motif Ligand 19; CK beta 11; CK beta-11; CKb11; EBI1 ligand chemokine; ELC; Epstein-Barr virus-induced molecule 1 ligand chemokine; Exodus 3; Macrophage inflammatory protein 3 beta; MGC34433; MIP 3 beta; MIP-3-beta; MIP-3B; MIP3 beta; MIP3BOTTHUMP00000000531; SCYA19; Small inducible cytokine A19; Small-inducible cytokine A19; CCL19 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CCL19
• Sequence Length: 98

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL19 elisa kit

Principle of the Assay: The Picokine™ Human CCL19 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CCL19 with a 96-well strip plate that is pre-coated with antibody specific for CCL19. The detection antibody is a biotinylated antibody specific for CCL19. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CCL19 with immunogen: Expression system for standard: E.coli; Immunogen sequence: G22-S98. The kit is analytically validated with ready to use reagents. To measure Human CCL19, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CCL19 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCL19 in the sample.!!Background/Introduction: Chemokine(C-C motif) ligand 19(CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine(ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

NCBI and Uniprot Product Information

• NCBI GI #: 74748724
• NCBI GeneID: 6363
• UniProt Accession #: Q6IBD6; Q99731
• Molecular Weight: 10,993 Da
• NCBI Official Full Name: CCL19 protein (Chemokine (C-C motif) ligand 19)
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 19
• NCBI Official Symbol: CCL19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; MIP-3b; SCYA19
• NCBI Protein Information: C-C motif chemokine 19; exodus-3; CK beta-11; MIP-3-beta; EBI1 ligand chemokine; EBI1-ligand chemokine; CC chemokine ligand 19; beta chemokine exodus-3; beta-chemokine exodus-3; small-inducible cytokine A19; macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; epstein-Barr virus-induced molecule 1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19
• UniProt Protein Name: C-C motif chemokine
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL19
• UniProt Entry Name: Q6IBD6_HUMAN

NCBI Description

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: extracellular space; extracellular region

Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; CCR7 chemokine receptor binding

Biological Process: formation of immunological synapse; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of JNK cascade; positive regulation of endocytosis; positive regulation of NF-kappaB import into nucleus; positive regulation of interleukin-1 beta secretion; activation of JNK activity; positive regulation of receptor-mediated endocytosis; dendritic cell chemotaxis; establishment of T cell polarity; positive regulation of T cell proliferation; cell communication; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of chemotaxis; response to virus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tumor necrosis factor production; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; T cell costimulation; immune response; positive regulation of T-helper 1 cell differentiation