Standard Curve (Sample)
Mouse Ovalbumin Specific IgE ELISA Kit | OVA sIgE elisa kit
Mouse Ovalbumin Specific IgE ELISA Kit
Reactivity
Mouse
Synonyms
Ovalbumin Specific IgE; Mouse Ovalbumin Specific IgE ELISA Kit; OVA sIgE; OVA sIgE elisa kit
Reactivity
Mouse
Specificity
Specifically recognize OVA sIgE, no obvious cross reaction with other analogues
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
1.563-100ng/ml
Sensitivity
0.938ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for OVA sIgE elisa kit
Background: OVA sIgE is a specific type of immunoglobulin E (IgE) antibody that targets Ovalbumin (OVA), a type of egg protein. sIgE stands for specific IgE antibody. sIgE is typically associated with allergic reactions. When the body is exposed to an allergen such as OVA, it stimulates the immune system to produce specific antibodies, including sIgE antibodies. These antibodies can activate mast cells and eosinophils, leading to inflammatory reactions and allergic symptoms. In medical diagnosis, testing for sIgE can be used to confirm allergic reactions caused by certain allergens. For example, testing for OVA sIgE can help confirm whether an individual is allergic to eggs in food allergies. Additionally, sIgE testing can be used to assess the severity and prognosis of allergic diseases such as asthma and urticaria.
Principle of the Assay: This kit was based on indirect enzyme-linked immune-sorbent assay technology. Antigen was pre-coated onto the 96-well plate. The HRP conjugated antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, HRP detection antibody was added to wells. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of target antigen in the sample is positively correlated with OD450 and can be calculated by plotting the standard curve.
Principle of the Assay: This kit was based on indirect enzyme-linked immune-sorbent assay technology. Antigen was pre-coated onto the 96-well plate. The HRP conjugated antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, HRP detection antibody was added to wells. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of target antigen in the sample is positively correlated with OD450 and can be calculated by plotting the standard curve.
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Product Notes
The Mouse OVA sIgE (Catalog #AAA17619) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17619 ELISA Kit recognizes Mouse OVA sIgE. It is sometimes possible for the material contained within the vial of "Ovalbumin Specific IgE, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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