Rabbit Tafazzin/TAZ Polyclonal Antibody | anti-TAZ antibody

Anti-Tafazzin/TAZ antibody

Cat. Num. AAA176048

• Gene Names: TAZ; EFE; BTHS; EFE2; G4.5; Taz1; CMD3A; LVNCX
• Reactivity: Reacts with: Human, Rat; Predicted to work with: Mouse
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: Tafazzin/TAZ; Polyclonal Antibody; Anti-Tafazzin/TAZ antibody; Tafazzin; tafazzin; Barth syndrome antibody; Cardiomyopathy dilated 3A(X linked) antibody; EFE2 antibody; Endocardial fibroelastosis 2 antibody; Protein G4.5 antibody; Tafazzin antibody; TAZ antibody; TAZ protein antibody; TAZ_HUMAN antibody; anti-TAZ antibody

• Host: Rabbit
• Reactivity: Reacts with: Human, Rat; Predicted to work with: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other species.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 292

• Applicable Applications for anti-TAZ antibody: Western Blot (WB)
• Application Notes: Western Blot (WB):; Concentration: 0.1-0.5ug/ml; Tested Species: Hu, Rat; Predicted Species: Mouse; ; Wester Blot: The detection limit for TAZ is approximately 10ng/lane under reducing condtions.; Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; Other applications have not been tested.; Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Tafazzin (162-178aa MDFILEKLNHGDWVHIF), identical to the related mouse and rat sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Tafazzin/TAZ antibody, MBS176048, Western blottingLane 1: Rat Skeletal Muscle Tissue LysateLane 2: Rat Heart Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: HELA Cell LysateLane 5: SMMC Cell LysateLane 6: SCG Cell Lysate )

Related Product Information for anti-TAZ antibody

Description: Rabbit IgG polyclonal antibody for Tafazzin(TAZ) detection. Tested with WB in Human, Mouse, Rat.
Background: Tafazzin, also known as G4.5 is a protein that in humans is encoded by the TAZ gene. Cardiolipin is a complex glycerophospholipid with 4 acyl groups that localizes to the mitochondrial inner membrane and has a role in mitochondrial structure and function. By positional cloning, TAZ was identified within the critical Barth syndrome region on Xq28. Tafazzin is involved in the metabolism of cardiolipin. It is a component of the hippo signaling pathway thatcontrols tissue growth in animals. And it can function as aphospholipid lysophospholipid transacylase.

NCBI and Uniprot Product Information

• NCBI GI #: 2498992
• NCBI GeneID: 6901
• UniProt Accession #: Q16635; Q5HY43; Q5HY44; Q5HY45; Q5HY48; Q86XQ6; Q86XQ7; Q86XQ8; Q86XQ9; Q86XR0; A3KQT2; D3DWX2
• Molecular Weight: 25,836 Da
• NCBI Official Full Name: Tafazzin
• NCBI Official Synonym Full Names: tafazzin
• NCBI Official Symbol: TAZ
• NCBI Official Synonym Symbols: EFE; BTHS; EFE2; G4.5; Taz1; CMD3A; LVNCX
• NCBI Protein Information: tafazzin; protein G4.5
• UniProt Protein Name: Tafazzin
• Protein Family: Tafazzin
• UniProt Gene Name: TAZ
• UniProt Synonym Gene Names: EFE2; G4.5
• UniProt Entry Name: TAZ_HUMAN

NCBI Description

This gene encodes a protein that is expressed at high levels in cardiac and skeletal muscle. Mutations in this gene have been associated with a number of clinical disorders including Barth syndrome, dilated cardiomyopathy (DCM), hypertrophic DCM, endocardial fibroelastosis, and left ventricular noncompaction (LVNC). Multiple transcript variants encoding different isoforms have been described. A long form and a short form of each of these isoforms is produced; the short form lacks a hydrophobic leader sequence and may exist as a cytoplasmic protein rather than being membrane-bound. Other alternatively spliced transcripts have been described but the full-length nature of all these transcripts is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

tafazzin: Some isoforms may be involved in cardiolipin (CL) metabolism. Defects in TAZ are the cause of Barth syndrome (BTHS). An X-linked disease characterized by dilated cardiomyopathy with endocardial fibroelastosis, a predominantly proximal skeletal myopathy, growth retardation, neutropenia, and organic aciduria, particularly excess of 3-methylglutaconic acid. Additional features include hypertrophic cardiomyopathy, isolated left ventricular non-compaction, ventricular arrhythmia, motor delay, poor appetite, fatigue and exercise intolerance, hypoglycemia, lactic acidosis, hyperammonemia, and dramatic late catch-up growth after growth delay throughout childhood. Belongs to the taffazin family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: mitochondrion; mitochondrial membrane; mitochondrial inner membrane; integral to membrane; intrinsic to membrane; cytosol; nucleus

Molecular Function: 1-acylglycerophosphocholine O-acyltransferase activity

Biological Process: cardiolipin biosynthetic process; cardiac muscle development; mitochondrial respiratory chain complex I assembly; skeletal muscle development; muscle contraction; cristae formation; phospholipid metabolic process; heart development; glycerophospholipid biosynthetic process; hemopoiesis; cardiac muscle contraction; organelle ATP synthesis coupled electron transport

Disease: Barth Syndrome