Western Blot (WB)
(Figure 1. Western blot analysis of FSTL3 using anti- FSTL3antibody (MBS176044).Lane 1: human U2OS whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human PC-3 whole cell lysatesLane 5: human HepG2 whole cell lysatesLane 6: human K562 whole cell lysates.)
Rabbit FSTL3 Polyclonal Antibody | anti-FSTL3 antibody
Anti-FSTL3 antibody
Western Blot (WB)
(Figure 1. Western blot analysis of FSTL3 using anti- FSTL3antibody (MBS176044).Lane 1: human U2OS whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human PC-3 whole cell lysatesLane 5: human HepG2 whole cell lysatesLane 6: human K562 whole cell lysates.)
Western Blot (WB)
(Figure 1. Western blot analysis of FSTL3 using anti- FSTL3antibody (MBS176044).Lane 1: human U2OS whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human PC-3 whole cell lysatesLane 5: human HepG2 whole cell lysatesLane 6: human K562 whole cell lysates.)
Western Blot (WB)
(Figure 2. Western blot analysis of FSTL3 using anti- FSTL3antibody (MBS176044).Lane 1: rat liver tissue lysatesLane 2: rat testis tissue lysatesLane 3: rat NRK whole cell lysatesLane 4: mouse liver tissue lysatesLane 5: mouse testis tissue lysatesLane 6: mouse lung tissue lysates.)
Western Blot (WB)
(Figure 2. Western blot analysis of FSTL3 using anti- FSTL3antibody (MBS176044).Lane 1: rat liver tissue lysatesLane 2: rat testis tissue lysatesLane 3: rat NRK whole cell lysatesLane 4: mouse liver tissue lysatesLane 5: mouse testis tissue lysatesLane 6: mouse lung tissue lysates.)
Background: FSTL3(Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins though to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. By Southern blot analysis of somatic cell hybrids and FISH, Hayette et al.(1998) localized the FSTL3 gene to chromosome 19p13. Using recombinant mouse Fstl3, Tsuchida et al.(2000) found that Fstl3bound both activin and BMP2 and had a higher affinity for activin. Overexpression of Fstl3 inhibited BMP2-induced cell signaling in a reporter assay. In addition to the translocation in a case of B-cell chronic lymphocytic leukemia from which FSTL3 was isolated, Hayette et al.(1998) also observed rearrangement of the FSTL3 gene in a case of B-cell non-Hodgkin lymphoma and in a case of B-cell mantle zone lymphoma, suggesting that FSTL3 may be involved in the leukemogenesis process.
NCBI and Uniprot Product Information
NCBI Description
Follistatin-like 3 is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
FSTL3: Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Oncoprotein; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19p13
Cellular Component: nucleoplasm; Golgi apparatus; extracellular space; extracellular region; nucleus; secretory granule
Molecular Function: protein binding; fibronectin binding; activin binding
Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of osteoclast differentiation; negative regulation of activin receptor signaling pathway; ossification; transcription, DNA-dependent; adrenal gland development; male gonad development; positive regulation of cell-cell adhesion; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; kidney development; negative regulation of BMP signaling pathway; lung development
Research Articles on FSTL3
Similar Products
Product Notes
The FSTL3 fstl3 (Catalog #AAA176044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FSTL3 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FSTL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the FSTL3 fstl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FSTL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
