Western Blot (WB)
(Anti-CD86 antibody, MBS175613, Western blottingLane 1: Recombinant Mouse CD86 Protein 10ngLane 2: Recombinant Mouse CD86 Protein 5ngLane 3: Recombinant Mouse CD86 Protein 2.5ng )
Rabbit anti-Mouse CD86 Polyclonal Antibody | anti-CD86 antibody
Anti-CD86 antibody
Gene Names
Cd86; B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
Reactivity
Mouse
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
CD86; Polyclonal Antibody; Anti-CD86 antibody; T-lymphocyte activation antigen CD86; CD86 molecule; Activation B7-2 antigen 3 antibody; Activation B7-2 antigen antibody; B-lymphocyte activation antigen B7-2 2 antibody; B-lymphocyte activation antigen B7-2 antibody; B7 2 antibody; B7 antibody; B7-2 antibody; B7.2 antibody; B70 antibody; B72 antigen antibody; BU63 antibody; CD28 antigen ligand 2 2 antibody; CD28 antigen ligand 2 antibody; Cd28l2 antibody; CD28LG2 antibody; CD86 antibody; CD86 antigen(CD28 antigen ligand 2; B7-2 antigen) 1; 2 antibody; B7-2 antigen) antibody; CD86 antigen antibody; CD86 molecule antibody; CD86_HUMAN antibody; CLS1 antibody; CTLA-4 counter-receptor B7.2 2; 3 antibody; CTLA-4 counter-receptor B7.2 antibody; Early T-cell costimulatory molecule 1 antibody; ETC-1 antibody; FUN 1 antibody; FUN-1 antibody; LAB72 antibody; Ly-58 antibody; Ly58 antibody; MB7 antibody; MB7-2 antibody; Membrane glycoprotein antibody; MGC34413 antibody; T-lymphocyte activation antigen CD86 antibody; TS/A-2 antibody; anti-CD86 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
309
Applicable Applications for anti-CD86 antibody
Western Blot (WB)
Application Notes
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: MS
Predicted Species: -
Antigen Retrieval: -
Tested Species: In-house tested species with positive results.
Predicted Species: Specices predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Concentration: 0.1-0.5ug/ml
Tested Species: MS
Predicted Species: -
Antigen Retrieval: -
Tested Species: In-house tested species with positive results.
Predicted Species: Specices predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CD86 (66-80aa YEHYLGTEKLDSVNA), different from the related rat sequence by two amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-CD86 antibody, MBS175613, Western blottingLane 1: Recombinant Mouse CD86 Protein 10ngLane 2: Recombinant Mouse CD86 Protein 5ngLane 3: Recombinant Mouse CD86 Protein 2.5ng )
Western Blot (WB)
(Anti-CD86 antibody, MBS175613, Western blottingLane 1: Recombinant Mouse CD86 Protein 10ngLane 2: Recombinant Mouse CD86 Protein 5ngLane 3: Recombinant Mouse CD86 Protein 2.5ng )
Related Product Information for anti-CD86 antibody
Description: Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD86(CD86) detection. Tested with WB in Mouse.
Background: CD86 is officially called Cluster of Differentiation 86, also known as B7-2. Being a member of the immunoglobulin superfamily, it is a protein expressed on antigen-presenting cells. The CD86 gene is mapped to human chromosome 3q21. The smallest transcript, 828 bp, which the authors termed CD86deltaTM, has a deletion from nucleotide 686 to nucleotide 829(i.e., exon 6) and encodes a 275-amino acid protein. CD86deltaTM enhances proliferation and cytokine production by both naive and memory T cells, providing costimulatory signals necessary for T cell activation and survival.
Background: CD86 is officially called Cluster of Differentiation 86, also known as B7-2. Being a member of the immunoglobulin superfamily, it is a protein expressed on antigen-presenting cells. The CD86 gene is mapped to human chromosome 3q21. The smallest transcript, 828 bp, which the authors termed CD86deltaTM, has a deletion from nucleotide 686 to nucleotide 829(i.e., exon 6) and encodes a 275-amino acid protein. CD86deltaTM enhances proliferation and cytokine production by both naive and memory T cells, providing costimulatory signals necessary for T cell activation and survival.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
33,962 Da
NCBI Official Full Name
T-lymphocyte activation antigen CD86
NCBI Official Synonym Full Names
CD86 antigen
NCBI Official Symbol
Cd86
NCBI Official Synonym Symbols
B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
NCBI Protein Information
T-lymphocyte activation antigen CD86; activation B7-2 antigen; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1
UniProt Protein Name
T-lymphocyte activation antigen CD86
Protein Family
UniProt Gene Name
Cd86
UniProt Synonym Gene Names
ETC-1
UniProt Entry Name
CD86_MOUSE
Uniprot Description
CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Cellular Component: cell surface; intracellular membrane-bound organelle; membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: receptor binding
Biological Process: B cell activation; negative regulation of T cell anergy; immune system process; toll-like receptor signaling pathway; positive regulation of T cell proliferation; positive regulation of activated T cell proliferation; defense response to virus
Research Articles on CD86
Similar Products
Product Notes
The CD86 cd86 (Catalog #AAA175613) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD86 antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD86 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: MS Predicted Species: - Antigen Retrieval: - Tested Species: In-house tested species with positive results. Predicted Species: Specices predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the CD86 cd86 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD86, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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