Western Blot (WB)
(Anti-HDJ2 antibody, MBS175545, Western blottingAll lanes: Anti HDJ2 (MBS175545) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Mouse Brain Tissue Lysate at 50ugLane 4: Mouse Lung Tissue Lysate at 50ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: A431 Whole Cell Lysate at 40ugLane 9: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD )
Rabbit HDJ2 Polyclonal Antibody | anti-DNAJA1 antibody
Anti-HDJ2 antibody
Gene Names
DNAJA1; DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
HDJ2; Polyclonal Antibody; Anti-HDJ2 antibody; DnaJ homolog subfamily A member 1; DnaJ(Hsp40) homolog; subfamily A; member 1; DJ 2 antibody; DJ2 antibody; DjA1 antibody; member 1 antibody; DnaJ homolog subfamily A member 1 antibody; DnaJ protein homolog 2 antibody; DNAJ2 antibody; Dnaja1 antibody; DNJA1_HUMAN antibody; hDJ 2 antibody; HDJ-2 antibody; HDJ2 antibody; Heat shock 40 kDa protein 4 antibody; heat shock protein DNAJ like 2 antibody; Heat shock protein J2 antibody; HSDJ antibody; HSJ-2 antibody; HSJ2 antibody; HSPF4 antibody; Human DnaJ protein 2 antibody; NEDD7 antibody; Neural precursor cell expressed developmentally down regulated 7 antibody; OTTHUMP00000021193 antibody; anti-DNAJA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
397
Applicable Applications for anti-DNAJA1 antibody
Western Blot (WB)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HDJ2 (369-384aa NQERRRHYNGEAYEDD), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-HDJ2 antibody, MBS175545, Western blottingAll lanes: Anti HDJ2 (MBS175545) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Mouse Brain Tissue Lysate at 50ugLane 4: Mouse Lung Tissue Lysate at 50ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: A431 Whole Cell Lysate at 40ugLane 9: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD )
Western Blot (WB)
(Anti-HDJ2 antibody, MBS175545, Western blottingAll lanes: Anti HDJ2 (MBS175545) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Mouse Brain Tissue Lysate at 50ugLane 4: Mouse Lung Tissue Lysate at 50ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: A431 Whole Cell Lysate at 40ugLane 9: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD )
Related Product Information for anti-DNAJA1 antibody
Description: Rabbit IgG polyclonal antibody for DnaJ homolog subfamily A member 1(DNAJA1) detection. Tested with WB in Human, Mouse, Rat.
Background: DNAJA1(DnaJ homolog subfamily A member 1), also called DJA1, HSDJ, HSPF4, is a protein that in humans is encoded by the DNAJA1 gene. The deduced 397-amino acid DNAJA1 protein is 32% identical to E. coli DnaJ, with the highest identity in the N-terminal region. Among the known DNAJ homologs in S. cerevisiae, DNAJA1 is most identical to YDJ1, which may be involved in the transport of certain proteins into the mitochondria and endoplasmic reticulum. Dja1 is expressed in mouse testis and prostate by immunohistochemical analysis and Western blot analysis. Transplantation experiments with fluorescence-tagged spermatogonia into Dja1 -/- mice revealed a primary defect of Sertoli cells in maintaining spermiogenesis at steps 8 and 9.
Background: DNAJA1(DnaJ homolog subfamily A member 1), also called DJA1, HSDJ, HSPF4, is a protein that in humans is encoded by the DNAJA1 gene. The deduced 397-amino acid DNAJA1 protein is 32% identical to E. coli DnaJ, with the highest identity in the N-terminal region. Among the known DNAJ homologs in S. cerevisiae, DNAJA1 is most identical to YDJ1, which may be involved in the transport of certain proteins into the mitochondria and endoplasmic reticulum. Dja1 is expressed in mouse testis and prostate by immunohistochemical analysis and Western blot analysis. Transplantation experiments with fluorescence-tagged spermatogonia into Dja1 -/- mice revealed a primary defect of Sertoli cells in maintaining spermiogenesis at steps 8 and 9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
37,045 Da
NCBI Official Full Name
DnaJ homolog subfamily A member 1
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily A, member 1
NCBI Official Symbol
DNAJA1
NCBI Official Synonym Symbols
DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2
NCBI Protein Information
dnaJ homolog subfamily A member 1; HSJ-2; human DnaJ protein 2; heat shock protein J2; dnaJ protein homolog 2; heat shock 40 kDa protein 4; heat shock protein, DNAJ-like 2; neural precursor cell expressed, developmentally down-regulated 7
UniProt Protein Name
DnaJ homolog subfamily A member 1
Protein Family
UniProt Gene Name
DNAJA1
UniProt Synonym Gene Names
DNAJ2; HDJ2; HSJ2; HSPF4; HSJ-2; hDj-2
UniProt Entry Name
DNJA1_HUMAN
Uniprot Description
DNAJA1: Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.
Protein type: Chaperone; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 9p13.3
Cellular Component: membrane; mitochondrion; perinuclear region of cytoplasm; nucleus; cytosol; ubiquitin ligase complex
Molecular Function: protein binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; ubiquitin protein ligase binding; chaperone binding; metal ion binding; unfolded protein binding; Hsp70 protein binding; ATP binding
Biological Process: regulation of protein transport; negative regulation of JNK activity; sperm motility; protein folding; positive regulation of apoptosis; response to heat; androgen receptor signaling pathway; DNA damage response, detection of DNA damage; negative regulation of protein ubiquitination; spermatogenesis; protein refolding; response to unfolded protein; negative regulation of apoptosis
Research Articles on DNAJA1
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Product Notes
The DNAJA1 dnaja1 (Catalog #AAA175545) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HDJ2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HDJ2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DNAJA1 dnaja1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDJ2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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