Rabbit DLGAP1 Polyclonal Antibody | anti-DLGAP1 antibody

Anti-DLGAP1 Antibody

Cat. Num. AAA1753784

• Gene Names: DLGAP1; GKAP; DAP-1; hGKAP; SAPAP1; DAP-1-BETA; DAP-1-ALPHA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: DLGAP1; Polyclonal Antibody; Anti-DLGAP1 Antibody; DAP1; GKAP; Disks large-associated protein 1; DAP-1; Guanylate kinase-associated protein; hGKAP; PSD-95/SAP90-binding protein 1; SAP90/PSD-95-associated protein 1; SAPAP1; DLG associated protein 1; anti-DLGAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for DLGAP1 detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-DLGAP1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml|Mouse, Rat|; IHC-P: 2-5ug/ml|Human, Rat|; ICC/IF: 5ug/ml|Human|; FC/FACS/FCM: 1-3ug/1x106 cells|Human|; Direct ELISA: 0.1-0.5ug/ml|Human|
• Immunogen: E Coli-derived human DLGAP1 recombinant protein (Position: D17-Q319).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-DLGAP1 antibody

Disks large-associated protein 1 (DAP-1), also known as guanylate kinase-associated protein (GKAP), is a protein that in humans is encoded by the DLGAP1 gene. The neurotransmitter glutamate facilitates neuronal signalling at excitatory synapses. Glutamate is released from the presynaptic membrane into the synaptic cleft. Across the synaptic cleft glutamate binds to both ion channels and metabotropic glutamate receptors at the postsynapse, which expedite downstream signalling in the neuron. The postsynaptic density, a highly specialized matrix, which is attached to the postsynaptic membrane, controls this downstream signalling. The postsynaptic density also resets the synapse after each synaptic firing. It is composed of numerous proteins including a family of Discs large associated protein 1, 2, 3 and 4 (DLGAP1-4) that act as scaffold proteins in the postsynaptic density. They link the glutamate receptors in the postsynaptic membrane to other glutamate receptors, to signalling proteins and to components of the cytoskeleton. With the central localisation in the postsynapse, the DLGAP family seems to play a vital role in synaptic scaling by regulating the turnover of both ionotropic and metabotropic glutamate receptors in response to synaptic activity. DLGAP family has been directly linked to a variety of psychological and neurological disorders.

References

1. Gross, M. B. Personal Communication. Baltimore, Md. 5/20/2014.
2. Kim, E., Naisbitt, S., Hsueh, Y. -P., Rao, A., Rothschild, A., Craig, A. M., Sheng, M. GKAP, a novel synaptic protein that interacts with the guanylate kinase-like domain of the PSD-95/SAP90 family of channel clustering molecules. J. Cell Biol. 136: 669-678, 1997.
3. Satoh, K., Yanai, H., Senda, T., Kohu, K., Nakamura, T., Okumura, N., Matsumine, A., Kobayashi, S., Toyoshima, K., Akiyama, T. DAP-1, a novel protein that interacts with the guanylate kinase-like domains of hDLG and PSD-95. Genes Cells 2: 415-424, 1997.

NCBI and Uniprot Product Information

• NCBI GI #: 51339031
• NCBI GeneID: 9229
• NCBI Accession #: NP_001003809.1
• NCBI GenBank Nucleotide #: NM_001003809.2
• UniProt Accession #: O14490; O14489; P78335; A8MWN8; B2RMU8; B7WPA1; B7Z2H2; B7Z2I2; B7Z9Y4
• Molecular Weight: 108,873 Da
• NCBI Official Full Name: disks large-associated protein 1 isoform 2
• NCBI Official Synonym Full Names: discs, large (Drosophila) homolog-associated protein 1
• NCBI Official Symbol: DLGAP1
• NCBI Official Synonym Symbols: GKAP; DAP-1; hGKAP; SAPAP1; DAP-1-BETA; DAP-1-ALPHA
• NCBI Protein Information: disks large-associated protein 1; PSD-95/SAP90 binding protein 1; SAP90/PSD-95-associated protein 1; guanylate kinase-associated protein
• UniProt Protein Name: Disks large-associated protein 1
• Protein Family: Disks large-associated protein
• UniProt Gene Name: DLGAP1
• UniProt Synonym Gene Names: DAP1; GKAP; DAP-1; hGKAP; SAPAP1
• UniProt Entry Name: DLGP1_HUMAN

Uniprot Description

SAPAP1: Part of the postsynaptic scaffold in neuronal cells. Belongs to the SAPAP family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 18p11.31

Cellular Component: postsynaptic membrane; cell junction

Molecular Function: protein binding

Biological Process: synaptic transmission