Rabbit SNAP23 Polyclonal Antibody | anti-SNAP23 antibody

Anti-SNAP23 antibody

Cat. Num. AAA175361

• Gene Names: SNAP23; SNAP-23; SNAP23A; SNAP23B; HsT17016
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: SNAP23; Polyclonal Antibody; Anti-SNAP23 antibody; Synaptosomal-associated protein 23(SNAP-23); synaptosomal-associated protein; 23kDa; HsT17016 antibody; LS-B8340 antibody; SNAP 23 antibody; SNAP-23 antibody; SNAP23 antibody; SNAP23A antibody; SNAP23B antibody; SNP23_HUMAN antibody; Synaptosomal associated protein 23 antibody; Synaptosomal associated protein 23kDa antibody; Synaptosomal associated protein antibody; Synaptosomal-associated protein 23 antibody; Vesicle membrane fusion protein SNAP 23 antibody; Vesicle membrane fusion protein SNAP23 antibody; Vesicle-membrane fusion protein SNAP-23 antibody; anti-SNAP23 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 211

• Applicable Applications for anti-SNAP23 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin
• Application Notes: Western Blot : Concentration: 0.1-0.5ug/mL; Tested Species: Human , Rat; Predicted Species: Mouse; ; Immunohistochemistry : Concentration: 0.5-1ug/mL; Tested Species : Human , Rat; Predicted Species: Mouse; Antigen Retrieval: By Heat; Immunohistochemistry (Frozen section) : Conentration: 0.5-1ug/mL ; Tested Species : Human, Mouse ; Predicted Species: Rat; ; Immunofluorescence/Immunocytochemsitry : Concentration: 5ug/mL ; Tested Species: Human; ; Flow Cytometry: Concentration: 1-3ug//1x10⁶ cells; Tested Species : Human; ; Other applications have not been tested; Optimal dilutions should be determined by end users.; ; Tested Species: In-house tested species with positive results; Predicted Species: Species predicted to be a fit for the product based on sequence similarities.; ; By Heat : Boililng the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 minutes is reqauired for the staining of formalin/parafin sections.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP23 (192-211aa DTNRDRIDIANARAKKLIDS), different from the related rat and mouse sequences by three amino acids.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-SNAP23 antibody, MBS175361, Western blottingLane 1: Rat Spleen Tissue LysateLane 2: Rat testis Tissue LysateLane 3: Rat Ovary Tissue LysateLane 4: HELA Cell LysateLane 5: MCF-7 Cell LysateLane 6: SKOV Cell Lysate )

Immunohistochemistry (IHC)

(Anti-SNAP23 antibody, MBS175361, IHC(P)IHC(P): Human Placenta Tissue )

Immunohistochemistry (IHC)

(Anti-SNAP23 antibody, MBS175361, IHC(F)IHC(F): Human Placenta Tissue )

Immunocytochemistry (ICC)

(Anti-SNAP23 antibody, MBS175361,ICCICC: K562 Cell )

Related Product Information for anti-SNAP23 antibody

Background: SNAP23(Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.

NCBI and Uniprot Product Information

• NCBI GI #: 6685971
• NCBI GeneID: 8773
• UniProt Accession #: O00161; O00162; Q13602; Q6IAE3
• NCBI Official Full Name: Synaptosomal-associated protein 23
• NCBI Official Synonym Full Names: synaptosomal-associated protein, 23kDa
• NCBI Official Symbol: SNAP23
• NCBI Official Synonym Symbols: SNAP-23; SNAP23A; SNAP23B; HsT17016
• NCBI Protein Information: synaptosomal-associated protein 23; synaptosomal-associated protein, 23kD; vesicle-membrane fusion protein SNAP-23
• UniProt Protein Name: Synaptosomal-associated protein 23
• Protein Family: Synaptosomal-associated protein
• UniProt Gene Name: SNAP23
• UniProt Synonym Gene Names: SNAP-23
• UniProt Entry Name: SNP23_HUMAN

NCBI Description

Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SNAP23: a member of the SNAP-25 family of SNARE proteins. Structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternatively spliced isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nucleoplasm; SNARE complex; azurophil granule; specific granule; neuron projection; mast cell granule; focal adhesion; cytoplasm; plasma membrane; synapse

Molecular Function: SNAP receptor activity; protein binding; syntaxin binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; protein transport; histamine secretion by mast cell; vesicle targeting; post-Golgi vesicle-mediated transport; synaptic vesicle priming