Western Blot (WB)
(Figure 1. Western blot analysis of PNN/DRSP using anti-PNN/DRSP antibody (MBS1753446).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human HL-60 whole cell lysatesLane 4: human K562 whole cell lysatesLane 5: rat liver tissue lysatesLane 6: rat kidney tissue lysatesLane 7: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PNN/DRSP antigen affinity purified polyclonal antibody (Catalog # MBS1753446) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PNN/DRSP at approximately 82KD. The expected band size for PNN/DRSPis at 82KD. )
Rabbit PNN/DRSP Polyclonal Antibody | anti-PNN antibody
Anti-PNN/DRSP Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of PNN/DRSP using anti-PNN/DRSP antibody (MBS1753446).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human HL-60 whole cell lysatesLane 4: human K562 whole cell lysatesLane 5: rat liver tissue lysatesLane 6: rat kidney tissue lysatesLane 7: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PNN/DRSP antigen affinity purified polyclonal antibody (Catalog # MBS1753446) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PNN/DRSP at approximately 82KD. The expected band size for PNN/DRSPis at 82KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of PNN/DRSP using anti-PNN/DRSP antibody (MBS1753446).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human HL-60 whole cell lysatesLane 4: human K562 whole cell lysatesLane 5: rat liver tissue lysatesLane 6: rat kidney tissue lysatesLane 7: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PNN/DRSP antigen affinity purified polyclonal antibody (Catalog # MBS1753446) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PNN/DRSP at approximately 82KD. The expected band size for PNN/DRSPis at 82KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-PNN/DRSP antibody (MBS1753446).Overlay histogram showing HepG2 cells stained with MBS1753446 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNN/DRSP Antibody (MBS1753446, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-PNN/DRSP antibody (MBS1753446).Overlay histogram showing HepG2 cells stained with MBS1753446 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNN/DRSP Antibody (MBS1753446, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of HL-60 cells using anti-PNN/DRSP antibody (MBS1753446).Overlay histogram showing HL-60 cells stained with MBS1753446 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNN/DRSP Antibody (MBS1753446, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of HL-60 cells using anti-PNN/DRSP antibody (MBS1753446).Overlay histogram showing HL-60 cells stained with MBS1753446 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNN/DRSP Antibody (MBS1753446, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Brandner, J. M., Reidenbach, S., Kuhn, C., Franke, W. W. Identification and characterization of a novel kind of nuclear protein occurring free in nucleoplasm and in ribonucleoprotein structures of the 'speckle' type. Europ. J. Cell Biol. 75: 295-308, 1998.
3. Degen, W. G. J., Agterbos, M. A., Muyrers, J. P. P., Bloemers, H. P. J., Swart, G. W. M. memA/DRS, a putative mediator of multiprotein complexes, is overexpressed in the metastasizing human melanoma cell lines BLM and MV3. Biochim. Biophys. Acta 1444: 384-394, 1999.
NCBI and Uniprot Product Information
Uniprot Description
PNN: Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma. Belongs to the pinin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Tumor suppressor; Spliceosome; RNA-binding; RNA splicing; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 14q21.1
Cellular Component: nucleoplasm; desmosome; membrane; cytoplasm; plasma membrane; intermediate filament; nuclear speck; intercellular junction
Molecular Function: DNA binding; structural molecule activity
Biological Process: nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of transcription, DNA-dependent; cell adhesion
Research Articles on PNN
Similar Products
Product Notes
The PNN pnn (Catalog #AAA1753446) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PNN/DRSP Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PNN/DRSP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.1-0.25ug/ml|Human, Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the PNN pnn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNN/DRSP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
