Rabbit IRAK-1/IRAK1 Polyclonal Antibody | anti-IRAK-1 antibody

Anti-IRAK-1/IRAK1 Antibody

Cat. Num. AAA1752848

• Gene Names: IRAK1; IRAK; pelle
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: IRAK-1/IRAK1; Polyclonal Antibody; Anti-IRAK-1/IRAK1 Antibody; Interleukin-1 receptor-associated kinase 1; IRAK-1; IRAK1; IRAK; anti-IRAK-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 682

• Applicable Applications for anti-IRAK-1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 0.25-0.5ug/mL; IHC-P: 0.5-1ug/mL; Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested.
• Immunogen: A synthetic peptide corresponding to a sequence of human IRAK-1/IRAK1 (NRNARVADLVHILTHLQLLRARD).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
• Relevant Detection Systems: MyBioSource recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P).
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, store at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-IRAK-1 antibody

Description: Rabbit IgG polyclonal antibody for IRAK-1/IRAK1 detection. Tested with WB, IHC-P in Human; Mouse; Rat.

Background: Interleukin-1 receptor-associated kinase 1, also called IRAK1, is an enzyme that in humans is encoded by the IRAK1 gene. By radiation hybrid analysis, this gene is mapped to chromosome Xq28. Serine/threonine-protein kinase plays a critical role in initiating innate immune response against foreign pathogens. This gene involved in Toll-like receptor (TLR) and IL-1R signaling pathways. This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.

References

1. Chassin, C., Kocur, M., Pott, J., Duerr, C. U., Gutle, D., Lotz, M., Hornef, M. W. miR-146a mediates protective innate immune tolerance in the neonate intestine. Cell Host Microbe 8: 358-368, 2010. 2. Jacob, C. O., Zhu, J., Armstrong, D. L., Yan, M., Han, J., Zhou, X. J., Thomas, J. A., Reiff, A., Myones, B. L., Ojwang, J. O., Kaufman, K. M., Klein-Gitelman, M., and 17 others. Identification of IRAK1 as a risk gene with critical role in the pathogenesis of systemic lupus erythematosus. Proc. Nat. Acad. Sci. 106: 6256-6261, 2009.

NCBI and Uniprot Product Information

• NCBI GI #: 68800343
• NCBI GeneID: 3654
• NCBI Accession #: NP_001020413.1
• NCBI GenBank Nucleotide #: NP_001020413.1
• UniProt Accession #: P51617
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 1 isoform 2
• NCBI Official Synonym Full Names: interleukin 1 receptor associated kinase 1
• NCBI Official Symbol: IRAK1
• NCBI Official Synonym Symbols: IRAK; pelle
• NCBI Protein Information: interleukin-1 receptor-associated kinase 1
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 1
• UniProt Gene Name: IRAK1
• UniProt Synonym Gene Names: IRAK; IRAK-1
• UniProt Entry Name: IRAK1_HUMAN

NCBI Description

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein type: Protein kinase, TKL; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); TKL group; IRAK family

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: interleukin-1 receptor complex; cytoplasm; plasma membrane; endosome membrane; lipid particle; nucleus; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; interleukin-1 receptor binding; protein heterodimerization activity; heat shock protein binding; ubiquitin-protein ligase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; protein amino acid autophosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; protein ubiquitination; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; positive regulation of MAP kinase activity; response to peptidoglycan; transmembrane receptor protein serine/threonine kinase signaling pathway; lipopolysaccharide-mediated signaling pathway; JNK cascade; toll-like receptor 4 signaling pathway; aging; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; activation of NF-kappaB-inducing kinase; protein oligomerization; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; toll-like receptor signaling pathway; innate immune response; regulation of cytokine and chemokine mediated signaling pathway; toll-like receptor 9 signaling pathway; negative regulation of apoptosis