Typical Testing Data/Standard Curve (for reference only)
Mouse sE-Selectin ELISA Kit | SELE elisa kit
Mouse sE-Selectin PicoKine Fast ELISA Kit
Gene Names
Sele; Elam; CD62E; ELAM-1; LECAM2; E-selectin
Reactivity
Mouse
Synonyms
sE-Selectin; Mouse sE-Selectin PicoKine Fast ELISA Kit; Mouse sE-Selectin; E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD62E; Sele; Elam-1; SELE elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Sele
Sequence Length
612
Samples
Cell culture supernates, serum and plasma(heparin, citrate)
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SELE elisa kit
Principle of the Assay: The Picokine™ Mouse Sele Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Sele with a 96-well strip plate that is pre-coated with antibody specific for Sele. The detection antibody is a biotinylated antibody specific for Sele. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Sele with immunogen: Expression system for standard: NSO; Immunogen sequence: W22-P557. The kit is analytically validated with ready to use reagents. To measure Mouse Sele, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Sele in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Sele in the sample.!!Background/Introduction: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissuespecific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
E-selectin
NCBI Official Synonym Full Names
selectin, endothelial cell
NCBI Official Symbol
Sele
NCBI Official Synonym Symbols
Elam; CD62E; ELAM-1; LECAM2; E-selectin
NCBI Protein Information
E-selectin
UniProt Protein Name
E-selectin
Protein Family
UniProt Gene Name
Sele
UniProt Synonym Gene Names
Elam-1; ELAM-1; LECAM2
UniProt Entry Name
LYAM2_MOUSE
Uniprot Description
SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family.
Protein type: Membrane protein, integral
Cellular Component: cortical cytoskeleton; extracellular space; membrane; perinuclear region of cytoplasm; integral to membrane; coated pit; caveola; lipid raft
Molecular Function: phospholipase binding; transmembrane receptor activity; carbohydrate binding; sialic acid binding
Biological Process: heterophilic cell adhesion; leukocyte adhesion; phospholipase C activation; positive regulation of leukocyte migration; positive regulation of receptor internalization; actin filament-based process; leukocyte tethering or rolling; cell adhesion; inflammatory response; signal transduction
Research Articles on SELE
Similar Products
Product Notes
The Mouse SELE sele (Catalog #AAA1752727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752727 ELISA Kit recognizes Mouse SELE. It is sometimes possible for the material contained within the vial of "sE-Selectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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