Rabbit GPR2/CCR10 Polyclonal Antibody | anti-CCR10 antibody

Anti-GPR2/CCR10 antibody

Cat. Num. AAA175255

• Gene Names: CCR10; GPR2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: GPR2/CCR10; Polyclonal Antibody; Anti-GPR2/CCR10 antibody; C-C chemokine receptor type 10(C-C CKR-10/CC-CKR-10/CCR-10); chemokine(C-C motif) receptor 10; C C chemokine receptor type 10 antibody; C C CKR 10 antibody; C-C chemokine receptor type 10 antibody; C-C CKR-10 antibody; CC chemokine receptor 10 antibody; CC CKR 10 antibody; CC-CKR-10 antibody; CCR 10 antibody; CCR-10 antibody; CCR10 antibody; CCR10_HUMAN antibody; Chemokine(C C motif) receptor 10 antibody; G protein coupled receptor 2 antibody; G protein-coupled receptor 2 antibody; G-protein coupled receptor 2 antibody; GPR 2 antibody; GPR2 antibody; anti-CCR10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 362

• Applicable Applications for anti-CCR10 antibody: Western Blot (WB)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GPR2/CCR10 (312-327aa FLGLRFRQDLRRLLRG), different from the related rat and mouse sequences by one amino acid.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-GPR2/CCR10 antibody, MBS175255, Western blottingLane 1: HELA Cell LysateLane 2: SW620 Cell LysateLane 3: A549 Cell LysateLane 4: MM231 Cell LysateLane 5: SMMC Cell Lysate )

Related Product Information for anti-CCR10 antibody

Description: Rabbit IgG polyclonal antibody for C-C chemokine receptor type 10(CCR10) detection. Tested with WB in Human, Mouse, Rat.
Background: CCR10, C-C chemokine receptor type 10 is a protein that in humans is encoded by the CCR10 gene. CCR10 is constitutively expressed in skin melanocytes, fibroblasts, and microvascular endothelial cells, and that it is upregulated by IL1B and TNF. Expression was also detected in T cells, Langerhans cells, and peripheral blood mononuclear cells but not in dendritic cells.By fluorescence in situ hybridization, the GPR2 gene is mapped to 17q21.1-q21.3. CCR10 is the receptor for CCL27. CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation. expression of CCL27 in normal keratinocytes and its strong upregulation in skin lesions of atopic dermatitis, contact dermatitis, and psoriasis patients. CCR10+ T lymphocytes were detected in lesional but not normal skin of these patients. Flow cytometric analysis showed that CCL27 binds extracellular matrix components and dermal microvascular endothelial cells and fibroblasts and mediates adhesion and transendothelial migration of CCR10+ circulating leukocytes. CCR10 is predominantly expressed on CD4+CLA+(cutaneous lymphocyte antigen), rather than CD8+, circulating T cells. RT-PCR, confocal microscopy, and ELISA analysis indicated that keratinocytes exposed to TNF or IL1B but not to IL4 or IFNG in vitro express increased CCL27.

NCBI and Uniprot Product Information

• NCBI GI #: 62298314
• NCBI GeneID: 2826
• UniProt Accession #: P46092; Q4V749; Q6T7X2; Q9NZG2
• Molecular Weight: 38,416 Da
• NCBI Official Full Name: C-C chemokine receptor type 10
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 10
• NCBI Official Symbol: CCR10
• NCBI Official Synonym Symbols: GPR2
• NCBI Protein Information: C-C chemokine receptor type 10; CCR-10; CC-CKR-10; C-C CKR-10; CC chemokine receptor 10; G protein-coupled receptor 2; G-protein coupled receptor 2
• UniProt Protein Name: C-C chemokine receptor type 10
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR10
• UniProt Synonym Gene Names: GPR2; C-C CKR-10; CC-CKR-10; CCR-10
• UniProt Entry Name: CCR10_HUMAN

NCBI Description

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CCR10 is the receptor for CCL27 (SCYA27; MIM 604833); CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (Homey et al., 2002 [PubMed 11821900]).[supplied by OMIM, Mar 2008]

Uniprot Description

CCR10: Receptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 17q21.1-q21.3

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; C-C chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; immune response; chemotaxis