Rabbit TAF12 Polyclonal Antibody | anti-TAF12 antibody

Anti-TAF12 Antibody

Cat. Num. AAA1751506

• Gene Names: TAF12; TAF2J; TAFII20
• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: TAF12; Polyclonal Antibody; Anti-TAF12 Antibody; Transcription initiation factor TFIID subunit 12; Transcription initiation factor TFIID 20/15 kDa subunits; TAFII-20; TAFII-15; TAFII20; TAFII15; TAF15; TAF2J; TATA-box binding protein associated factor 12; anti-TAF12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized
• Sequence Length: 131

• Applicable Applications for anti-TAF12 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 0.1-0.5ug/ml; IHC-P: 0.5-1ug/ml ; Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested.
• Immunogen: A synthetic peptide corresponding to a sequence of human TAF12 (QIADDFIESVVTAACQLARHRKSSTLEVKDVQLHLERQ).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
• Relevant Detection Systems: We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P).
• Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, store at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.; Avoid repeated freezing and thawing.

Related Product Information for anti-TAF12 antibody

Description: Rabbit IgG polyclonal antibody for TAF12 detection. Tested with WB, IHC-P in Human; Mouse; Rat.
Background: Transcription initiation factor TFIID subunit 12 is a protein that in humans is encoded by the TAF12 gene. Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene.

References

1. Bieniossek, C., Papai, G., Schaffitzel, C., Garzoni, F., Chaillet, M., Scheer, E., Papadopoulos, P., Tora, L., Schultz, P., Berger, I. The architecture of human general transcription factor TFIID core complex. Nature 493: 699-702, 2013. 2. Mengus, G., May, M., Jacq, X., Staub, A., Tora, L., Chambon, P., Davidson, I. Cloning and characterization of hTAFII18, hTAFII20 and hTAFII28: three subunits of the human transcription factor TFIID. EMBO J. 14: 1520-1531, 1995.

NCBI and Uniprot Product Information

• NCBI GI #: 206725450
• NCBI GeneID: 6883
• NCBI Accession #: NP_001128690.1
• NCBI GenBank Nucleotide #: NM_001135218.1
• UniProt Accession #: Q16514; Q15775; Q5T077; D3DPM5
• Molecular Weight: 14,825 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 12
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor 12
• NCBI Official Symbol: TAF12
• NCBI Official Synonym Symbols: TAF2J; TAFII20
• NCBI Protein Information: transcription initiation factor TFIID subunit 12
• UniProt Protein Name: Transcription initiation factor TFIID subunit 12
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF12
• UniProt Synonym Gene Names: TAF15; TAF2J; TAFII20; TAFII-20/TAFII-15; TAFII20/TAFII15

NCBI Description

Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription.