Avidin Active Protein | AVD active protein
Avidin, Egg White - >10 U/mg protein
Purity
Highly Purified
Synonyms
Avidin; Egg White - >10 U/mg protein; Egg White; >=10 U/mg Protein; AVD active protein
Purity/Purification
Highly Purified
Form/Format
Lyophilized
Sequence Length
152
Application Notes
Application: Egg white Avidin can be used in assays for functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Source
Chicken Egg White
Moisture
< 5%
EC Number
215-783-6
Unit Definition
Amount of Avidin that will bind one microgram of d-biotin.
Recertification
2 years
Protein
>= 0.90 mg/mg solid
Moisture Content
< 5%
Contaminants
Ash: < 0.1%
CAS Number
1405-69-2
Activity
>= 10 U/mg solid
Preparation and Storage
2-8 degree C
Related Product Information for AVD active protein
MyBioSource produces proteins from chicken egg whites, including Albumin, Lysozyme and Avidin, for research, life science and manufacturing applications.
Custom preparations, technical support, bulk quantities and aliquoting available.
Avidin is a tetrameric protein. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer) each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.
Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including Western Blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.
Avidin and biotin are routinely conjugated to indicator enzymes, such as alkaline phosphatase and horseradish peroxidase for use in kit development and manufacturing.
Custom preparations, technical support, bulk quantities and aliquoting available.
Avidin is a tetrameric protein. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer) each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.
Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including Western Blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.
Avidin and biotin are routinely conjugated to indicator enzymes, such as alkaline phosphatase and horseradish peroxidase for use in kit development and manufacturing.
Product Categories/Family for AVD active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66kDa with subunit at 16kDa
NCBI Official Full Name
avidin
NCBI Official Synonym Full Names
avidin
NCBI Official Symbol
AVD
NCBI Protein Information
avidin; mature avidin
UniProt Protein Name
Avidin
Protein Family
UniProt Gene Name
AVD
UniProt Entry Name
AVID_CHICK
Uniprot Description
The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
Research Articles on AVD
Similar Products
Product Notes
The AVD avd (Catalog #AAA173235) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. Application: Egg white Avidin can be used in assays for functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol. Researchers should empirically determine the suitability of the AVD avd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Avidin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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