Horse Collagen Type lV ELISA Kit | COL4 elisa kit

Horse Collagen Type lV ELISA Kit

Cat. Num. AAA17290

• Gene Names: COL6A1; OPLL
• Reactivity: Horse
• Synonyms: Collagen Type lV; Horse Collagen Type lV ELISA Kit; COL4 elisa kit

• Reactivity: Horse
• Sequence Length: 1028

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 6.25 ng/ml ~ 200 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Horse COL4 in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Horse COL4 concentration. The concentration of Horse COL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 87196339
• NCBI GeneID: 1291
• NCBI Accession #: NP_001839.2
• NCBI GenBank Nucleotide #: NM_001848.2
• UniProt Accession #: P12109; O00117; O00118; Q14040; Q14041; Q16258; Q7Z645; Q9BSA8
• Molecular Weight: 108,529 Da
• NCBI Official Full Name: collagen alpha-1(VI) chain
• NCBI Official Synonym Full Names: collagen, type VI, alpha 1
• NCBI Official Symbol: COL6A1
• NCBI Official Synonym Symbols: OPLL
• NCBI Protein Information: collagen alpha-1(VI) chain; collagen alpha-1(VI) chain; alpha 1 (VI) chain (61 AA); collagen VI, alpha-1 polypeptide
• UniProt Protein Name: Collagen alpha-1(VI) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL6A1
• UniProt Entry Name: CO6A1_HUMAN

NCBI Description

The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

COL6A1: Collagen VI acts as a cell-binding protein. Defects in COL6A1 are a cause of Bethlem myopathy (BM). BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles. Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD); also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. Belongs to the type VI collagen family.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; protein complex; membrane; lysosomal membrane; endoplasmic reticulum lumen; extracellular region; sarcolemma; collagen type VI

Molecular Function: platelet-derived growth factor binding

Biological Process: osteoblast differentiation; axon guidance; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cell adhesion

Disease: Bethlem Myopathy; Ullrich Congenital Muscular Dystrophy