Bovine Collagen Type X ELISA Kit | COL10 elisa kit

Bovine Collagen Type X ELISA Kit

Cat. Num. AAA17154

• Reactivity: Bovine
• Synonyms: Collagen Type X; Bovine Collagen Type X ELISA Kit; COL10 elisa kit

• Reactivity: Bovine
• Specificity: This kit recognizes recombinant and natural Bovine COL10, no significant cross-reactivity or interference was observed.
• Sequence Length: 680

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 3.12 ng/ml ~ 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Bovine COL10 in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Bovine COL10 concentration. The concentration of Bovine COL10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL10 elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 119568625
• NCBI GeneID: 1300
• UniProt Accession #: Q03692; A1L4P2
• Molecular Weight: 66,158 Da
• NCBI Official Full Name: collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia)
• NCBI Official Synonym Full Names: collagen, type X, alpha 1
• NCBI Official Symbol: COL10A1
• NCBI Protein Information: collagen alpha-1(X) chain; collagen alpha-1(X) chain; collagen X, alpha-1 polypeptide; Schmid metaphyseal chondrodysplasia
• UniProt Protein Name: Collagen alpha-1(X) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL10A1
• UniProt Entry Name: COAA1_HUMAN

NCBI Description

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]

Uniprot Description

COL10A1: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage. Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD). SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q21-q22

Cellular Component: proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; extracellular region; cell cortex

Molecular Function: metal ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; cartilage development; skeletal development; endochondral ossification

Disease: Metaphyseal Chondrodysplasia, Schmid Type