Human Oxysterol-binding protein-related protein 3, OSBPL3 ELISA Kit | OSBPL3 elisa kit

Human Oxysterol-binding protein-related protein 3, OSBPL3 ELISA Kit

Cat. Num. AAA166705

• Gene Names: OSBPL3; ORP3; ORP-3; OSBP3
• Reactivity: Human
• Synonyms: Oxysterol-binding protein-related protein 3; OSBPL3; Human Oxysterol-binding protein-related protein 3; OSBPL3 ELISA Kit; OSBPL3 elisa kit

• Reactivity: Human
• Sequence Length: 851

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.1ng/ml - 38ng/ml
• Sensitivity: 0.053ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OSBPL3 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Oxysterol-binding Protein-related Protein 3 (also known as OSBPL3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human OSBPL3 antibody. OSBPL3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human OSBPL3 Antibody is added and binds to OSBPL3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated OSBPL3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human OSBPL3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 21735582
• NCBI GeneID: 26031
• NCBI Accession #: NP_663161.1
• NCBI GenBank Nucleotide #: NM_145321.2
• UniProt Accession #: Q9H4L5; O14591; O43357; O43358; A4D167; A4D168; A4D169; A4D170; A4D171; A4D172; B8ZZ79; B8ZZP0
• Molecular Weight: 97,687 Da
• NCBI Official Full Name: oxysterol-binding protein-related protein 3 isoform c
• NCBI Official Synonym Full Names: oxysterol binding protein like 3
• NCBI Official Symbol: OSBPL3
• NCBI Official Synonym Symbols: ORP3; ORP-3; OSBP3
• NCBI Protein Information: oxysterol-binding protein-related protein 3
• UniProt Protein Name: Oxysterol-binding protein-related protein 3
• Protein Family: Oxysterol-binding protein-related protein
• UniProt Gene Name: OSBPL3
• UniProt Synonym Gene Names: KIAA0704; ORP3; OSBP3; ORP-3; OSBP-related protein 3

NCBI Description

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. The encoded protein is involved in the regulation of cell adhesion and organization of the actin cytoskeleton. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes (PubMed:16143324). Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments (PubMed:25447204). The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed:18270267, PubMed:25447204). With VAPA, may regulate ER morphology (PubMed:16143324). Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion (PubMed:18270267). Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3 (PubMed:16143324). Also binds 25-hydroxycholesterol and cholesterol (PubMed:17428193).