Human leucocyte antigen B27, HLA-B27 ELISA Kit | HLA-B27 elisa kit

Human leucocyte antigen B27, HLA-B27 ELISA Kit

Cat. Num. AAA165358

• Gene Names: HLA-B; AS; HLAB; B-4901
• Reactivity: Human
• Synonyms: leucocyte antigen B27; HLA-B27; Human leucocyte antigen B27; HLA-B27 ELISA Kit; HLA-B27 elisa kit

• Reactivity: Human
• Sequence Length: 362

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.5-300ng/ml
• Sensitivity: 0.32ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HLA-B27 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human leucocyte antigen B27 (also known as HLA-B27) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human HLA-B27 antibody. HLA-B27 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HLA-B27 Antibody is added and binds to HLA-B27 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HLA-B27 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HLA-B27. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 32177
• NCBI GeneID: 3106
• UniProt Accession #: P03989; O19692; P10317; P10318; P19373; P30467; Q08136; Q29693; Q29846; Q29961; P01889; P10319; P18463; P18464; P18465; P30460; P30461; P30462; P30464
• Molecular Weight: 40,428 Da
• NCBI Official Full Name: HLA-B27, partial
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, B
• NCBI Official Symbol: HLA-B
• NCBI Official Synonym Symbols: AS; HLAB; B-4901
• NCBI Protein Information: major histocompatibility complex, class I, B
• UniProt Protein Name: HLA class I histocompatibility antigen, B-27 alpha chain
• UniProt Gene Name: HLA-B
• UniProt Synonym Gene Names: HLAB

NCBI Description

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.